20.500.12556/RUL-145178 Design, development, and testing of a device for gene electrotransfer to skin cells in vivo Gene electrotransfer (GET) is considered one of the most efficient, safe, reproducible, and cost-effective methods of gene therapy, in which a gene is delivered to the cells in the form of a plasmid DNA vector by a method known as electroporation. To achieve successful electroporation, cells must be exposed to sufficiently high electric fields generated by short-duration, high-voltage electrical pulses that result in a temporary increase in plasma membrane permeability. The electrical pulses are generated by pulse generators (electroporators) and delivered to the cells via electrodes (applicators). However, there is a lack of standardized pulse delivery protocols as well as certified clinical pulse generators and applicators for gene delivery. In this paper, the development of a new pulse generator, applicator, and pulse delivery protocol for GET to skin cells is presented. A numerical model of electroporated skin developed and tested for two electrode configurations and two different pulse delivery protocols is also presented. An alternative pulse delivery protocol was proposed. The developed pulse generator, applicator, and the proposed pulse delivery protocol were then used in vivo for GET to skin cells in mice. The results showed high efficiency of the proposed pulse delivery protocol for the purpose of GET in mouse skin cells. Specifically, electroporation with the developed pulse generator, applicator, and proposed pulse delivery protocol resulted in higher gene expression in skin cells compared to the currently used pulse generator, applicator, and pulse delivery protocol. electroporation gene electrotransfer GET plasmid DNA pulse generator pulse delivery protocol elektroporacija elektroprenos genov plazmidna DNK true false true Angleški jezik Slovenski jezik Članek v reviji 2023-04-12 12:33:04 2023-04-12 12:33:06 2023-04-13 03:31:25 0000-00-00 00:00:00 2022 0 0 14 str. iss. 9, art. 1826 Vol. 14 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602.6/.7 1999-4923 10.3390/pharmaceutics14091826 119715587 pharmaceutics-14-01826.pdf pharmaceutics-14-01826.pdf 1 7FA952F334326587B6063176A4C10A0C 7b985ebf9ac0419c0175e6abe199b05acc549d45dbe5f0b6dd58a523b9177fd6 9d4eb8ea-d91d-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167877 https://www.mdpi.com/1999-4923/14/9/1826 1 44ba52bd-d91d-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167876 Fakulteta za elektrotehniko 0 0 0