20.500.12556/RUL-145170 Ceramide phosphoethanolamine as a possible marker of periodontal disease Periodontal disease is a chronic oral inflammatory disorder initiated by pathobiontic bacteria found in dental plaques—complex biofilms on the tooth surface. The disease begins as an acute local inflammation of the gingival tissue (gingivitis) and can progress to periodontitis, which eventually leads to the formation of periodontal pockets and ultimately results in tooth loss. The main problem in periodontology is that the diagnosis is based on the assessment of the already obvious tissue damage. Therefore, it is necessary to improve the current diagnostics used to assess periodontal disease. Using lipidomic analyses, we show that both crucial periodontal pathogens, Porphyromonas gingivalis and Tannerella forsythia, synthesize ceramide phosphoethanolamine (CPE) species, membrane sphingolipids not typically found in vertebrates. Previously, it was shown that this particular lipid can be specifically detected by an aegerolysin protein, erylysin A (EryA). Here, we show that EryA can specifically bind to CPE species from the total lipid extract from P. gingivalis. Furthermore, using a fluorescently labelled EryA-mCherry, we were able to detect CPE species in clinical samples of dental plaque from periodontal patients. These results demonstrate the potential of specific periodontal pathogen-derived lipids as biomarkers for periodontal disease and other chronic inflammatory diseases. ceramide phosphoethanolamine periodontal disease aegerolysin biomarker erylysin A Porphyromonas gingivalis ceramid fosfoetanolamin parodontalna bolezen egerolizin true false true Angleški jezik Slovenski jezik Članek v reviji 2023-04-12 10:12:55 2023-04-12 10:13:00 2023-04-13 03:31:25 0000-00-00 00:00:00 2022 0 0 19 str. iss. 7, art. 655 Vol. 12 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.31 2077-0375 10.3390/membranes12070655 113667587 membranes-12-00655-v2.pdf membranes-12-00655-v2.pdf 1 627EC322E1DF06510778542C2261F799 8a83d024f1c5f7e58ba104744d182156a8378fcb4548803062887c0f9bb17de0 a2a8f912-d90a-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167861 https://www.mdpi.com/2077-0375/12/7/655 1 b2f099ae-d909-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167860 Biotehniška fakulteta Medicinska fakulteta 0 0 0