20.500.12556/RUL-145027 Genomic insights into antibiotic resistance and mobilome of lactic acid bacteria and bifidobacteria Lactic acid bacteria (LAB) and Bifidobacterium sp. (bifidobacteria) can carry antimicrobial resistance genes (ARGs), yet data on resistance mechanisms in these bacteria are limited. The aim of our study was to identify the underlying genetic mechanisms of phenotypic resistance in 103 LAB and bifidobacteria using whole-genome sequencing. Sequencing data not only confirmed the presence of 36 acquired ARGs in genomes of 18 strains, but also revealed wide dissemination of intrinsic ARGs. The presence of acquired ARGs on known and novel mobile genetic elements raises the possibility of their horizontal spread. In addition, our data suggest that mutations may be a common mechanism of resistance. Several novel candidate resistance mechanisms were uncovered, providing a basis for further in vitro studies. Overall, 1,314 minimum inhibitory concentrations matched with genotypes in 92.4% of the cases; however, prediction of phenotype based on genotypic data was only partially efficient, especially with respect to aminoglycosides and chloramphenicol. Our study sheds light on resistance mechanisms and their transferability potential in LAB and bifidobacteria, which will be useful for risk assessment analysis. genomics functional genomics microbiology virology host pathogen interaction mikorbiologija bakterije mlečnokislinske bakterije genetika genomika odpornost true false true Angleški jezik Slovenski jezik Članek v reviji 2023-03-30 13:47:33 2023-03-30 13:47:34 2024-08-27 15:52:29 0000-00-00 00:00:00 2023 0 0 15 str. no. 4, art. e202201637 Vol. 6 2023 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 579:575 2575-1077 10.26508/lsa.202201637 141893123 e202201637.full.pdf e202201637.full.pdf 1 C64E1E27BF3597D29640378CAFCED43A e8aaff5723441d8d32ea08bf2f37a5a4cb7e511f11f13daad61d21a5d7ba5c7d 54a149ca-8ae2-11ee-8840-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=177966 https://www.life-science-alliance.org/content/6/4/e202201637 1 912247f9-cef0-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167650 Biotehniška fakulteta 0 0 0