20.500.12556/RUL-145025 Clonal spread of pESI-positive multidrug-resistant ST32 Salmonella enterica serovar Infantis isolates among broilers and humans in Slovenia Salmonella enterica subsp. enterica serovar Infantis is the most prevalent serovar found in broilers and broiler meat and is among the top five serovars responsible for human infections in Europe. In 2008, a multidrug-resistant S. Infantis isolate emerged in Israel with a mosaic megaplasmid named pESI, associated with increased virulence, biofilm formation, and multidrug resistance. Since then, S. Infantis clones with pESI-like plasmids have been reported worldwide, replacing pESI-free clones. Here, we typed 161 S. Infantis isolates of poultry (n = 133) and human clinical (n = 28) origin using whole-genome sequencing. The isolates were collected between 2007 and 2021. In addition, we performed PacBio/Illumina sequencing for two representative pESI-like plasmids and compared them with publicly available sequences. All isolates belonged to sequence type 32 (ST32), except for one isolate that represented a novel single-locus variant of ST32. Core genome MLST (cgMLST) analysis revealed 14 clusters of genetically closely related isolates, of which four suggested broiler-to-human transmission of S. Infantis. pESI-like plasmids were present in 148/161 (91.9%) isolates; all were highly similar to the publicly available pESI-like sequences but lacked extended-spectrum beta-lactamase (ESBL) genes. PacBio/Illumina hybrid assembly allowed the reconstruction of two novel complete pESI variants. The present study revealed that the multidrug-resistant, pESI-positive S. Infantis clone became the predominant S. Infantis clone in Slovenian broilers and humans during the last decade. Continued surveillance of resistant S. Infantis clones along the food chain is needed to guide public health efforts. Salmonella Infantis ST32 pESI multidrug resistance broilers humans true false true Angleški jezik Ni določen Članek v reviji 2023-03-30 13:03:57 2023-03-30 13:04:06 2023-03-31 03:36:29 0000-00-00 00:00:00 2022 0 0 9 str. no. 6, [article no.] e02481-22 Vol. 10 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09:579 2165-0497 10.1128/spectrum.02481-22 130438147 Clonal_spread-Avbersek.pdf Clonal_spread-Avbersek.pdf 1 97F8F9C58256861C6BE256C793628A10 8792ddd412dbb68f8dc6dd69ef500836f949bb36242f21fae9aa216cd86d9c7e 9551d92d-ceeb-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167647 https://journals.asm.org/doi/10.1128/spectrum.02481-22 1 7c5faf7e-ceea-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167646 Veterinarska fakulteta 0 0 0