20.500.12556/RUL-144774 Modulacija adhezije bakterij Campylobacter in Listeria na modelnih površinah Adhesion of bacteria Campylobacter and Listeria on model surfaces Patogene bakterije iz rodu Campylobacter in Listeria so bakterije prenosljive s hrano, ki so sposobne adhezije na različnih inertnih površinah uporabljenih v industriji, kar predstavlja prvo fazo pri nastanku biofilma. Biofilm celice zaščiti pred neugodnimi pogoji, ter jim omogoči preživetje in obstojnost v prehranjevalni verigi, kjer lahko pride do navzkrižne kontaminacije in prenosa do naslednjega gostitelja. Svetovna zdravstvena organizacija poroča o porastu pojavnosti kampilobakterioze in listerioze kot posledice uživanja nezadostno obdelanega kontaminiranega mesa, mleka ali vode. V magistrskem delu smo ovrednotili adhezijo bakterij vrst Campylobacter jejuni, Listeria monocytogenes in Listeria innocua na: (i) modelni abiotski površini iz polistirena prisotnega v prehrambenih obratih, (ii) na modelnih biotskih površinah kot so mucin, fibronektin in kolagen, ki so prisotni v humanem prebavnem traktu. V nadaljevanju smo ovrednotili adhezijo na biotske ter abiotske površine po dodatku 10 lektinov iz gob. Rezultati so pokazali različno oziroma specifično sposobnost adhezije med bakterijami Campylobacter ter Listeria, ter tudi večjo sposobnost adhezije na biotske površine. Izpostavimo lahko lektina Tectonin2 (bakterije C. jejuni) in ABL (bakterije L. monocytogenes in L. innocua), ki predstavljata potencialno učinkovino naravnega izvora ter usmerjata razvoj novih strategij nadzora nad patogenimi bakterijami preko zmanjševanja oziroma preprečevanja adhezije. Pathogenic bacteria of the genus Campylobacter and Listeria are foodborne bacteria capable of adhering to various inert surfaces used in industry, which is the first stage of biofilm formation. The biofilm protects the cells from adverse conditions and allows them to survive and remain in the food chain, where cross-contamination and transmission to the next host can occur. The World Health Organization reports an increase in campylobacteriosis and listeriosis as a result of consumption of inadequately processed contaminated meat, milk or water. In the Master thesis, we investigated the adhesion of Campylobacter jejuni, Listeria monocytogenes and Listeria innocua on: (i) abiotic model surfaces of polystyrene found in food crops, (ii) biotic model surfaces of mucin, fibronectin and collagen found in the human digestive tract. We also investigated the modulation of adhesion to biotic and abiotic surfaces after addition of 10 fungal lectins. The results showed a different/specific ability of adhesion between Campylobacter and Listeria, and a greater ability of adhesion on biotic surfaces. Thus, the lectins Tectonin2 (C. jejuni) and ABL (L. monocytogenes in L. innocua) represent effective agents of natural origin that allow the development of new control strategies against pathogenic bacteria by reducing/preventing adhesion. Campylobacter jejuni Listeria monocytogenes Listeria innocua patogene bakterije adhezija lektini modelna abiotska površina modelna biotska površina polistiren mucin fibronektin kolagen Campylobacter jejuni Listeria monocytogenes Listeria innocua pathogenic bacteria adhesion lectins model abiotic surface model biotic surface polystyrene mucin fibronectin collagen true false false [M. Pesterean] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2023-03-12 07:15:20 2023-03-12 07:15:26 2023-03-13 12:35:57 0000-00-00 00:00:00 2023 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.24/.26:547.96 217550 144942595 32122.pdf 32122.pdf 1 EE6926168FEC175281E8D7B189826F29 c335ff63b82584529d7c42479347af116a9169bc1f98498488e840ca79bbe85c 3b9bf905-c09d-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167283 Biotehniška fakulteta 0 0 0