20.500.12556/RUL-144758 Astrocyte arborization enhances Ca$^{2+}$ but not cAMP signaling plasticity The plasticity of astrocytes is fundamental for their principal function, maintaining homeostasis of the central nervous system throughout life, and is associated with diverse exposomal challenges. Here, we used cultured astrocytes to investigate at subcellular level basic cell processes under controlled environmental conditions. We compared astroglial functional and signaling plasticity in standard serum-containing growth medium, a condition mimicking pathologic conditions, and in medium without serum, favoring the acquisition of arborized morphology. Using opto−/electrophysiologic techniques, we examined cell viability, expression of astroglial markers, vesicle dynamics, and cytosolic Ca$^{2+}$ and cAMP signaling. The results revealed altered vesicle dynamics in arborized astrocytes that was associated with increased resting [Ca$^{2+}$]$_i$ and increased subcellular heterogeneity in [Ca$^{2+}$]$_i$, whereas [cAMP]$_i$ subcellular dynamics remained stable in both cultures, indicating that cAMP signaling is less prone to plastic remodeling than Ca$^{2+}$ signaling, possibly also in in vivo contexts. astrocytes vesicles confocal microscopy Ca$^{2+}$ cAMP electrophysiology astrociti vezikli konfokalna mikroskopija true false true Angleški jezik Slovenski jezik Članek v reviji 2023-03-10 13:27:05 2023-03-10 13:27:09 2023-03-11 03:36:49 0000-00-00 00:00:00 2021 0 0 Str. 2899-2916 iss. 12 Vol. 69 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-092 0894-1491 10.1002/glia.24076 73561091 Glia_-_2021_-_Pirnat_-_Astrocyte_arborization_enhances_Ca2__but_not_.pdf Glia_-_2021_-_Pirnat_-_Astrocyte_arborization_enhances_Ca2__but_not_.pdf 1 2F30CF94CEB8E20E453F021E30E24668 b3ae8a97f7d9387fc7d79327dc997c5c339026247169eafa1c01b0ee97ab83ad 12f0cc42-bf3f-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167257 https://onlinelibrary.wiley.com/doi/10.1002/glia.24076 0 eeace764-bf3e-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167256 Medicinska fakulteta 0 0 0