20.500.12556/RUL-144674 Antimicrobial activity of human fetal membranes from biological function to clinical use The fetal membranes provide a supportive environment for the growing embryo and later fetus. Due to their versatile properties, the use of fetal membranes in tissue engineering and regenerative medicine is increasing in recent years. Moreover, as microbial infections present a crucial complication in various treatments, their antimicrobial properties are gaining more attention. The antimicrobial peptides (AMPs) are secreted by cells from various perinatal derivatives, including human amnio-chorionic membrane (hACM), human amniotic membrane (hAM), and human chorionic membrane (hCM). By exhibiting antibacterial, antifungal, antiviral, and antiprotozoal activities and immunomodulatory activities, they contribute to ensuring a healthy pregnancy and preventing complications. Several research groups investigated the antimicrobial properties of hACM, hAM, and hCM and their derivatives. These studies advanced basic knowledge of antimicrobial properties of perinatal derivatives and also provided an important insight into the potential of utilizing their antimicrobial properties in a clinical setting. After surveying the studies presenting assays on antimicrobial activity of hACM, hAM, and hCM, we identified several considerations to be taken into account when planning future studies and eventual translation of fetal membranes and their derivatives as antimicrobial agents from bench to bedside. Namely, (1) the standardization of hACM, hAM, and hCM preparation to guarantee rigorous antimicrobial activity, (2) standardization of the antimicrobial susceptibility testing methods to enable comparison of results between various studies, (3) investigation of the antimicrobial properties of fetal membranes and their derivatives in the in vivo setting, and (4) designation of donor criteria that enable the optimal donor selection. By taking these considerations into account, future studies will provide crucial information that will enable reaching the optimal treatment outcomes using the fetal membranes and their derivatives as antimicrobial agents. fetal membrane perinatal derivatives amniotic membrane amnio-chorionic membrane placenta antimicrobial peptides bacteria antibacterial activity plodna membrana perinatalni derivati amnijska membrana true false true Angleški jezik Slovenski jezik Članek v reviji 2023-03-07 15:17:08 2023-03-07 15:17:11 2023-03-08 03:33:19 0000-00-00 00:00:00 2021 0 0 16 str. Vol. 9, art. 691522 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 618.2/.7 2296-4185 10.3389/fbioe.2021.691522 65347075 fbioe-09-691522.pdf fbioe-09-691522.pdf 1 377A91291709A1C61D105CECC0D2C75A b04d5c7a1c22d3414a4ef43311a6986b19b7ea93347850f73db608e52f48fe82 fecb650f-bcf2-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167135 https://www.frontiersin.org/articles/10.3389/fbioe.2021.691522/full 1 b941330d-bcf2-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167134 Medicinska fakulteta Biotehniška fakulteta 0 0 0