20.500.12556/RUL-144646 The antibacterial effects of new N-alkylpyridinium salts on planktonic and biofilm bacteria An increasing microbial resistance to known antibiotics raises a demand for new antimicrobials. In this study the antimicrobial properties of a series of new N-Alkylpyridinium quaternary ammonium compounds (QACs) with varying alkyl chain lengths were evaluated for several nosocomial pathogens. The chemical identities of the new QACs were determined by NMR, LC-MS, and HRMS. All the planktonic bacteria tested were susceptible to the new QACs as evaluated by MIC and MBC assays. The antimicrobial effect was most pronounced against Staphylococcus aureus clinical isolates. Live/dead staining CLSM was used to test the effectiveness of the QACs in biofilms. The effectiveness was up to 10-fold lower than in the plankton. When QACs were used as irrigants in Er:YAG – SSP photoacoustic steaming, their effectiveness significantly increased. The combined use of irrigants and photoacoustic streaming increased biofilm removal from the surface and increased the killing rate of the cells remaining on the surface. This may allow for a shorter chemical exposure time and lower dosage of QACs used in applications. The results demonstrate that the new QACs have potential to be applied as antibacterial compounds effective against planktonic and biofilm bacteria as well as irrigants in removal of difficult-to-reach biofilms. antimicrobial activity quaternary ammonium salts bacteria biofilm photoacoustic irrigation biofilmi bakterijski biofilmi Staphylococcus aureus kvarterne amonijeve spojine protimikrobna aktivnost true false true Angleški jezik Slovenski jezik Članek v reviji 2023-03-06 14:06:47 2023-03-06 14:06:48 2023-03-07 03:26:42 0000-00-00 00:00:00 2020 0 0 12 str. Vol. 11, art. 573951 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.6 1664-302X 10.3389/fmicb.2020.573951 33768963 fmicb-11-573951.pdf fmicb-11-573951.pdf 1 0D816D3EDB0E0882216AF9C073227DA4 ed3aea1ccb3e4237f3170aaf64e98d8c4293dd5f87958d7c1215a97ae406eddc 18e181b4-bc21-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167085 https://www.frontiersin.org/articles/10.3389/fmicb.2020.573951/full 0 bbb26d6c-bc20-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167084 Biotehniška fakulteta 0 0 0