20.500.12556/RUL-144465 Small cellular particles from European spruce needle homogenate Small cellular particles (SCPs) are being considered for their role in cell-to-cell communication. We harvested and characterized SCPs from spruce needle homogenate. SCPs were isolated by differential ultracentrifugation. They were imaged by scanning electron microscope (SEM) and cryogenic transmission electron microscope (cryo TEM), assessed for their number density and hydrodynamic diameter by interferometric light microscopy (ILM) and flow cytometry (FCM), total phenolic content (TPC) by UV-vis spectroscopy, and terpene content by gas chromatography-mass spectrometry (GC-MS). The supernatant after ultracentrifugation at 50,000× g contained bilayer-enclosed vesicles whereas in the isolate we observed small particles of other types and only a few vesicles. The number density of cell-sized particles (CSPs) (larger than 2 μm) and meso-sized particles (MSPs) (cca 400 nm–2 µm) was about four orders of magnitude lower than the number density of SCPs (sized below 500 nm). The average hydrodynamic diameter of SCPs measured in 10,029 SCPs was 161 ± 133 nm. TCP decreased considerably due to 5-day aging. Volatile terpenoid content was found in the pellet after 300× g. The above results indicate that spruce needle homogenate is a source of vesicles to be explored for potential delivery use. Picea abies spruce needles extracellular particles extracellular vesicles small cellular particles interferometric light microscopy exosomes cell-to-cell communication drug delivery Picea abies smrekove iglice zunajcelični delci zunajcelični vezikli majhni celični delci interferometrična svetlobna mikroskopija eksosomi komunikacija med celicami dostava zdravil true false true Angleški jezik Ni določen Članek v reviji 2023-02-23 08:34:53 2023-02-23 08:34:55 2023-03-10 07:43:03 0000-00-00 00:00:00 2023 0 0 16 str. no. 5, art. 4349 Vol. 24 Feb. 2023 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2023-02-22 577:615.3 1422-0067 10.3390/ijms24054349 142622467 10.3390_ijms24054349.pdf 10.3390_ijms24054349.pdf 1 C2FAC4B5BAFF7F36BDB75F03DDF43094 3c65f2fa2b36520a12f31b26faf59d4eb57f4a052825668c9e4068d03a5e3bdf db0a84d8-b35f-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=166794 https://www.mdpi.com/1422-0067/24/5/4349 1 39efa9e1-b34c-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=166784 Zdravstvena fakulteta Fakulteta za elektrotehniko Fakulteta za kemijo in kemijsko tehnologijo Medicinska fakulteta 0 0 0