20.500.12556/RUL-144338 ATR-FTIR spectroscopy and chemometric complexity unfolding the intra-skeleton variability Spectroscopy of skeletal tissues is increasingly used in various fields, including legal medicine, forensic science, and archaeology. As it is fast and technically and financially affordable yet accurate and widely applicable, spectroscopy is often used when investigating skeletal tissues. Despite its usefulness, the heterogeneity of skeletal tissues highly affects their chemical composition and complicates the interpretation of the spectroscopy results. Though the research on the use of spectroscopy and skeletal tissues from various contexts is growing, little is known regarding the differences in the chemometric indices caused by intra-skeleton variability. Objectives The objectives were a comparison of the chemometric indices between teeth and bones, between different bone classes, and between the skeletal elements within a bone class as well as an attempt to correlate the observed similarities or differences in chemometric indices to the functional or structural differences of skeletal elements. Materials and Methods Different skeletal elements of three individuals were analysed with attenuated total reflectance (ATR)–Fourier Transform Infrared (FTIR) spectroscopy and compared. Results Significant differences between the chemometric indices of the bones and teeth were observed and meaningfully correlated with their structural differences. Though more subtle and harder to understand, significant differences were also observed between and within the bone classes and were tentatively correlated with their structural and functional differences. Discussion The observed variability agrees with other studies that stress the importance of intra-skeleton variability, which should be acknowledged when using spectroscopy to investigate skeletal tissues. ATR-FTIR spectroscopy intra-skeletal variability chemometrics bone type skeletal element ATR-FTIR spektroskopija znotrajskeletna variabilnost kemometrija true false true Angleški jezik Slovenski jezik Članek v reviji 2023-02-15 10:57:43 2023-02-15 10:57:48 2023-02-16 03:41:16 0000-00-00 00:00:00 2022 0 0 15 str. iss. 11, art. e3448 Vol. 36 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 61 1099-128X 10.1002/cem.3448 123441923 Journal_of_Chemometrics_-_2022_-_Zupani__Pajni_-_ATR‐.pdf Journal_of_Chemometrics_-_2022_-_Zupani__Pajni_-_ATR‐.pdf 1 C1ED2F05B56CF6FDB26232F9B61E19AA 90591018425b9e198c0a594f115dd81572e81851b7ac2cb3fe7cd2e9c6ff8e95 1f0e5a93-ad17-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=166604 https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/cem.3448 0 06092a58-ad17-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=166603 Filozofska fakulteta Medicinska fakulteta 0 0 0