20.500.12556/RUL-144325 Advances in physicochemical and biochemical characterization of archaeosomes from polar lipids of Aeropyrum pernix K1 and stability in biological systems Archaeosomes are vesicles made from archaeal lipids. They are characterized by remarkable thermostability, resistance to enzymatic degradation, long-term stability, and immunomodulatory properties. In this review the current status of physicochemical properties of archaeal lipids and their stability in biological systems is presented, focusing on total polar lipids from Aeropyrum pernix K1. The isolated total polar lipids from Aeropyrum pernix K1 consist exclusively of glycerol ether lipids with isoprenoid groups attached to glycerol via ether linkages. More specifically, the two major polar lipids extracted from the membranes are C$_{25,25}$-achaetidyl(glucosyl)inositol and C$_{25,25}$-achaetidylinositol. An overview of the results of the effects of temperature and pH on the stability, structural organization, fluidity, and permeability of archaeosomes composed of pure C$_{25,25}$ was examined by a combination of techniques, including fluorescence emission spectroscopy, electron paramagnetic resonance, differential scanning calorimetry, and confocal microscopy. We also compared the physicochemical properties of pure vesicles composed of either archaeal lipids or conventional lipids (e.g., 1,2-dipalmitoyl-sn-glycero-3-phosphocholine) with mixed vesicles composed of both lipid types. Archaeal lipids are discussed in terms of their potential use as a targeted drug delivery system based on the results of in vivo and cytotoxicity studies. cells lipids membranes stability vesicles liposomi arheosomi arheje lipidi arhej fizikalnokemijske lastnosti dostavni sistemi true false true Angleški jezik Slovenski jezik Članek v reviji 2023-02-14 14:43:07 2023-02-14 14:43:08 2023-02-15 03:41:00 0000-00-00 00:00:00 2023 0 0 Str. 2861-2870 iss. 3 Vol. 8 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.35:582.233:615.014.6 2470-1343 10.1021/acsomega.2c07406 138234627 acsomega.2c07406.pdf acsomega.2c07406.pdf 1 747E8AD7BD8D6047B72CD454AD09F409 70b82a7ef4099e5159b21845651d84623f8ea9c627de49a01e3a3d8cc565cdc0 6900d3d6-ac6d-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=166577 https://pubs.acs.org/doi/10.1021/acsomega.2c07406 1 305dd0f9-ac6d-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=166576 Biotehniška fakulteta 0 0 0