20.500.12556/RUL-144312 Bacterial DNA recognition by SERS active plasma-coupled nanogold It is shown that surface-enhanced Raman spectroscopy (SERS) can identify bacteria based on their genomic DNA composition, acting as a “sample-distinguishing marker”. Successful spectral differentiation of bacterial species was accomplished with nanogold aggregates synthesized through single-step plasma reduction of the ionic gold-containing vapored precursor. A high enhancement factor (EF = 10$^7$) in truncated coupled plasmonic particulates allowed SERS-probing at nanogram sample quantities. Simulations confirmed the occurrence of the strongest electric field confinement within nanometric gaps between gold dimers/chains from where the molecular fingerprints of bacterial DNA fragments gained photon scattering enhancement. The most prominent Raman modes linked to fundamental base-pair molecular vibrations were deconvoluted and used to proceed with nitrogenous base content estimation. The genomic composition (percentage of guanine-cytosine and adenine-thymine) was successfully validated by third-generation sequencing using nanopore technology, further proving that the SERS technique can be employed to swiftly specify bioentities by the discriminative principal-component statistical approach. DNA genomic ration plasma electrochemical reduction coupled plasmonic nanogold DNA Raman fingerprints genetics genomics gold plasma Raman spectroscopy true false true Angleški jezik Ni določen Članek v reviji 2023-02-14 10:11:00 2023-02-14 10:11:00 2023-02-22 11:12:21 0000-00-00 00:00:00 2022 0 0 Str. 9757-9765 iss. 23 Vol. 22 Dec. 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 533 1530-6992 10.1021/acs.nanolett.2c02835 127669763 acs.nanolett.2c02835.pdf acs.nanolett.2c02835.pdf 1 4DF80D733F2C955522D612510C40840E b71941c1d80cc4526979aab22b3bd2f8ad25b0bc1be17f7cddff9ea88cf439b0 5a0d724a-ac47-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=166557 https://pubs.acs.org/doi/10.1021/acs.nanolett.2c02835 0 4a2c475a-ac47-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=166556 Medicinska fakulteta 0 0 0