20.500.12556/RUL-144172 Antimicrobial and antibiofilm effect of commonly used disinfectants on Salmonella Infantis isolates Salmonella enterica subsp. enterica serovar Infantis is the most prevalent serovar in broilers and broiler meat in the European Union. The aim of our study was to test the biofilm formation and antimicrobial effect of disinfectants on genetically characterized S. Infantis isolates from poultry, food, and humans. For the biofilm formation under various temperature conditions (8 °C, 20 °C, and 28 °C) and incubation times (72 h and 168 h), the crystal violet staining method was used. The evaluation of the in vitro antimicrobial effect of Ecocid® S, ethanol, and hydrogen peroxide was determined using the broth microdilution method. The antibiofilm effect of subinhibitory concentration (1/8 MIC) of disinfectants was then tested on S. Infantis 323/19 strain that had the highest biofilm formation potential. Our results showed that the biofilm formation was strain‐specific; however, it was higher at 20 °C and prolonged incubation time. Moreover, strains carrying a pESI plasmid showed higher biofilm formation potential. The antibiofilm potential of disinfectants on S. Infantis 323/19 strain at 20 °C was effective after a shorter incubation time. As shown in our study, more effective precautionary measures should be implemented to ensure biofilm prevention and removal in order to control the S. Infantis occurrence. Salmonella Infantis biofilm antibiofilm disinfectants true false true Angleški jezik Ni določen Članek v reviji 2023-02-02 11:48:54 2023-02-02 11:48:55 2023-02-04 03:18:50 0000-00-00 00:00:00 2023 0 0 12 str. iss. 2, [article no.] 301 Vol. 11 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09:579 2076-2607 10.3390/microorganisms11020301 140082435 Antimicrobial_and_antibiofilm-Zorman.pdf Antimicrobial_and_antibiofilm-Zorman.pdf 1 470758A3FE0ECEC63DB91C489C185DEC 0beab5d6f2021208ccb155d1b79a8a928f68984a3ff4800760ebbdf0822fb9fa 22115fe3-a2e9-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=166352 https://www.mdpi.com/2076-2607/11/2/301 1 e6b6be88-a2e6-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=166346 Veterinarska fakulteta 0 0 0