20.500.12556/RUL-144108 Biochemical characterization of black and green mutant elderberry during fruit ripening The content of sugars, organic acids, phenolic compounds and selected enzyme activities in the anthocyanin pathway were analyzed in NIGRA (Sambucus nigra var. nigra—black fruits) and VIRIDIS (S. nigra var. viridis—green fruits) fruits over four stages of ripening. The share of glucose and fructose in green fruits was higher than in colored fruits, and the sugar content increased significantly until the third developmental stage. Ripe NIGRA berries had 47% flavonol glycosides, 34% anthocyanins, 3% hydroxycinnamic acids and 14% flavanols, whereas the major phenolic group in the VIRIDIS fruits, making up 88% of the total analyzed polyphenols, was flavonols. NIGRA fruits were rich in anthocyanins (6020 [mi]g g$^{-1}$ FW), showing strong activation of the late anthocyanin pathway (dihydroflavonol 4-reductase, anthocyanidin synthase). In both color types, phenylalanine ammonia lyase and chalcone synthase/chalcone isomerase activities were highest in the first stage and decreased during ripening. In VIRIDIS fruit, no anthocyanins and only one flavanol (procyanidin dimer) were found. This was most likely caused by a lack of induction of the late anthocyanin pathway in the last period of fruit ripening. The VIRIDIS genotype may be useful in studying the regulatory structures of anthocyanin biosynthesis and the contribution of distinct flavonoid classes to the health benefits of elderberries. black elderberry Sambucus nigra green genotype polyphenolics sugars organic acids phenylpropanoid enzymes črni bezeg zeleni genotip polifenoli sladkorji organske kisline fenilpropanoidni encimi true false true Angleški jezik Slovenski jezik Članek v reviji 2023-02-01 11:33:48 2023-02-01 11:33:56 2023-02-03 03:11:09 0000-00-00 00:00:00 2023 0 0 16 str. iss. 3, art. 504 Vol. 12 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 634.1/.7 2223-7747 10.3390/ plants12030504 140263683 plants-12-00504.pdf plants-12-00504.pdf 1 3CF136D3C0F48467AFD035304C35FFE8 e49191b029c160981f794938681087c45dd133a2ed347ee06de04d50a7cacf8e dd38cc43-a21d-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=166248 https://www.mdpi.com/2223-7747/12/3/504 1 a75b4155-a21b-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=166247 Biotehniška fakulteta 0 0 0