20.500.12556/RUL-144061 Towards the development of a biorelevant in vitro method for the prediction of nanoemulsion stability on the ocular surface Ophthalmic oil-in-water nanoemulsions (NEs) are a complex technological platform, representing an advancement in the treatment of dry eye disease. In addition to enabling the incorporation of poorly soluble active pharmaceutical ingredients (APIs), NEs provide prolonged residence time of APIs and other formulation components and consequent replenishment and stabilization of the compromised tear film. Ophthalmic NEs have been on the market for over 20 years, but considering their complexity, as well as the complex nature of the ocular surface, they are still a poorly understood advanced dosage form. The objective of this study was to develop a biorelevant in vitro method that would be able to predict the behavior of ophthalmic NEs after application. With that goal, NE formulations differing in critical material attributes and critical formulation variables were employed and subjected to simulated tear turnover and blinking. By gradually increasing the complexity of the in vitro method, we were able to detect key parameters influencing NE stability. The undertaken study presents a step forward in the development of in vitro tools that are fundamental to the reliable, cost and time-effective development of innovative and generic topical ophthalmic NEs. eye drops nanoemulsion chitosan dilution high shear dynamic light scattering kapljice za oko nanoemulzije dinamično sipanje svetlobe visoko striženje true false true Angleški jezik Slovenski jezik Članek v reviji 2023-01-30 11:01:31 2023-01-30 11:01:36 2023-01-31 03:47:53 0000-00-00 00:00:00 2023 0 0 11 str. Vol. 633 art. 122622 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.017-022.513.2:620.3 0378-5173 10.1016/j.ijpharm.2023.122622 139859459 1-s2.0-S037851732300042X-main.pdf 1-s2.0-S037851732300042X-main.pdf 1 C7A2505BA3BD124C2CF0A7738DF77B07 329e8be09dde95d346d6229b68efa4f0b798168f3ee239306d57c94cea731bb9 584d565f-a085-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=166187 https://www.sciencedirect.com/science/article/pii/S037851732300042X?via%3Dihub 1 d0bcda4e-a084-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=166186 Fakulteta za farmacijo 0 0 0