20.500.12556/RUL-143958 Characterization of the Slovene autochthonous rose hybrid Rosa pendulina × spinosissima (Rosa reversa Waldst. and Kit) using biochemical patterns of the plant blossoms The Rosa genus is characterized by great variability and, consequently, they easily hybridize. The petals of R. pendulina, R. spinosissima and their hybrid Rosa pendulina x spinosissima, collected in western Slovenia, were included in the research. We performed morphometric analysis using keys to determine roses and genetic analysis to determine the genome size. The phenolic compound content in petals of all rose flowers was measured by liquid chromatography and mass spectrometry (HPLC-MS). Using flow cytometry, we could confirm the native hybridization process due to the amount of 2C DNA. The value of R. pendulina was 1.71 pg, of R. spinosissima 1.60 pg and of the hybrid 1.62 pg. The value for the hybrid was close to values measured for parent plants and, at the same time, those values of parent plants significantly differed from each other. Our results showed that the content of phenolic compounds in petals decreased after crossing. We found that the highest total phenolic content (178.9 g/kg FW) was measured in R. spinossisima, the lowest content was analyzed for the hybrid (84.36 g/kg FW) and the content for R. pendulina was between these two values (110.58 g/kg FW). The content of flavanols and flavonols was lowest in the hybrid petals, whereas the content of anthocyanins was highest in the hybrid petals. autochthonous rose HPLC-MS flow cytometry hybrids phenols Rosa pendulina Rosa spinosissima Rosa pendulina x spinosissima domača vrnica pretočna citometrija fenoli true false true Angleški jezik Slovenski jezik Članek v reviji 2023-01-24 08:13:00 2023-01-24 08:13:07 2024-02-16 15:27:18 0000-00-00 00:00:00 2023 0 0 16 str. iss. 3, art. 505 Vol. 12 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 665.5 2223-7747 10.3390/plants12030505 139079171 plants-12-00505.pdf plants-12-00505.pdf 1 CEE5FB802F9952CFF1197E7AC99A3429 63960b344f40fab44e485ff6e1adfb857ed155b5af0fc3c4a4e7d1247ef4dd77 bf9b3447-9bb6-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=166038 https://www.mdpi.com/2223-7747/12/3/505 1 4cdc5f57-9bb6-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=166037 Biotehniška fakulteta 0 0 0