20.500.12556/RUL-143705 Terpenoids as natural agents against food-borne bacteria - evaluation of biofilm biomass versus viability reduction This study aimed to analyse the antibacterial potential of limonene, terpineol, and eugenol for the biofilm reduction of food-borne E. coli, S. aureus and S. typhimurium. A microdilution test with resazurin application was used for the minimum inhibitory concentration and a colony plate count was used for the minimum bactericidal concentration. Biofilm biomass was quantified using the crystal violet assay, while biofilm viability was determined using the plate count method. The results show the highest antibacterial potential among terpenoids for eugenol, followed by terpineol and limonene. Both biomass reduction and viability are strongly dependent on the concentration of all terpenoids tested (p < 0.05). Moreover, eugenol reduced biofilm biomass most effectively (67% for E. coli), while viability was reduced most by terpineol (3.8 log CFU cm−2 for E. coli and S. aureus). The correlation coefficient for the reduction in biomass and viability was highest for eugenol (0.9) and chlorhexidine for all bacteria tested, while the lowest correlation was found for limonene (0.6). Results also demonstrate that tested terpenoids are effective as standard antimicrobial agent chlorhexidine. This suggests that eugenol has potential against food-borne biofilms as it simultaneously reduces both biomass and viability of biofilms terpenoids food-borne bacteria biofilm minimum inhibitory concentration viability biomass terpenoidi bakterije ki se prenašajo s hrano biofilm najmanjša inhibitorna koncentracija sposobnost preživetja biomasa true false true Angleški jezik Ni določen Članek v reviji 2023-01-10 07:09:59 2023-01-10 07:10:01 2023-01-11 03:28:05 0000-00-00 00:00:00 2023 0 0 12 str. iss. 1, art. 148 Vol. 11 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2023-01-03 614.31 2227-9717 10.3390/pr11010148 136896515 Terpenoids_as_Natural_Agents_against_Food-Borne_Bacteria.pdf Terpenoids_as_Natural_Agents_against_Food-Borne_Bacteria.pdf 1 FE731458EFC541FAFE8F32641011C921 a694d2c30445b4b310c829625e0278bb39ee620e2e93ac33760ddeb951682a7e 896ded9f-90ad-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=165584 https://www.mdpi.com/2227-9717/11/1/148 1 3070d827-90ad-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=165583 Zdravstvena fakulteta 0 0 0