20.500.12556/RUL-143699 Assessment of small cellular particles from four different natural sources and liposomes by interferometric light microscopy Small particles in natural sources are a subject of interest for their potential role in intercellular, inter-organism, and inter-species interactions, but their harvesting and assessment present a challenge due to their small size and transient identity. We applied a recently developed interferometric light microscopy (ILM) to assess the number density and hydrodynamic radius (Rh) of isolated small cellular particles (SCPs) from blood preparations (plasma and washed erythrocytes) (B), spruce needle homogenate (S), suspension of flagellae of microalgae Tetraselmis chuii (T), conditioned culture media of microalgae Phaeodactylum tricornutum (P), and liposomes (L). The aliquots were also assessed by flow cytometry (FCM), dynamic light scattering (DLS), ultraviolet-visible spectrometry (UV-vis), and imaging by cryogenic transmission electron microscopy (cryo-TEM). In Rh, ILM showed agreement with DLS within the measurement error in 10 out of 13 samples and was the only method used here that yielded particle density. Cryo-TEM revealed that representative SCPs from Tetraselmis chuii flagella (T) did not have a globular shape, so the interpretation by Rh of the batch methods was biased. Cryo-TEM showed the presence of thin filaments in isolates from Phaeodactylum tricornutum conditioned culture media (P), which provides an explanation for the considerably larger Rh obtained by batch methods than the sizes of particles observed by cryo-TEM images. ILM proved convenient for assessment of number density and Rh of SCPs in blood preparations (e.g., plasma); therefore, its use in population and clinical studies is indicated. interferometric light microscopy interferometric nanotracking analysis nanoparticle tracking analysis concentration of extracellular particles size of extracellular particles dynamic light scattering hydrodynamic radius of extracellular particles extracellular vesicles liposomes exosomes interferometrična svetlobna mikroskopija interferometrična nanosledilna analiza analiza sledenja nanodelcev koncentracija zunajceličnih delcev velikost zunajceličnih delcev dinamično sipanje svetlobe hidrodinamični radij zunajceličnih delcev zunajcelični vezikli liposomi eksosomi true false true Angleški jezik Ni določen Članek v reviji 2023-01-09 12:25:12 2023-01-09 12:25:14 2023-01-10 03:29:56 0000-00-00 00:00:00 2022 0 0 22 str. no. 24, art. 15801 Vol. 23 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2022-12-13 577:543.456 1422-0067 10.3390/ijms232415801 136801795 ijms-23-15801-v2.pdf ijms-23-15801-v2.pdf 1 7717AC84080CDCD2FAAFFC95EA205EC1 ae05f65c6b19ed7f701b69c0c092d2c86be095832420a7093025716cba4cbaa7 408426e5-9011-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=165567 https://www.mdpi.com/1422-0067/23/24/15801 1 0fab9216-9010-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=165565 Zdravstvena fakulteta Fakulteta za elektrotehniko Fakulteta za kemijo in kemijsko tehnologijo Medicinska fakulteta 0 0 0