20.500.12556/RUL-143570 Bisphenol A in edible tissues of rams exposed to repeated low-level dietary dose by high-performance liquid chromatography with fluorescence detection The presented work deals with levels and distribution of bisphenol A (BPA) in the edible tissues of a large food-producing animal species. An experimental animal study included 14 young Istrian pramenka rams (Ovis aries), of which seven were exposed for 64 days to a low dietary dose of BPA at 25 µg/kg b.w./day, and seven served as a control group. Residue analysis of both aglycone and total BPA was performed in the muscle tissue, liver, kidney and fat tissue of the individual animals by means of enzymatic deconjugation (for total BPA), organic solvent extraction, molecularly imprinted polymer solid-phase extraction (MISPE) clean-up and high-performance liquid chromatography with fluorescence detection (HPLC-FLU). The analysis was optimized and validated for aglycone BPA in the fat tissue and for the total BPA in all tissues investigated. Edible tissues of the control group of rams generally remained BPA-free, while there were concentration differences between the control and treated groups for liver and kidney post last administration. The human health risk resulting from this study was assessed by the estimated dietary exposure in adults, which was < 0.1% related to the valid European Union Tolerable Daily Intake (TDI) value of 4 µg/kg b.w./day. However, it would be 58-fold higher than the newly proposed TDI value of 0.04 ng/kg b.w./day. Bisphenol A Total bisphenol A Sheep Tissues Distribution HPLC-fluorescence Risk assessment true false true Angleški jezik Angleški jezik Članek v reviji 2022-12-28 10:16:45 2022-12-28 10:16:46 2022-12-29 03:30:26 0000-00-00 00:00:00 2022 0 0 Str. 76078-76090 no. 50 Vol. 29 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09:615.9 1614-7499 10.1007/s11356-022-21154-5 110581763 Bisphenol.pdf Bisphenol.pdf 1 189463BFA26166F5D3C3A23B43155959 d47b4a94f4c489011edff814c2bfd220458eae663a7eea68b63c5daedc36056d c19b368d-8692-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=165304 https://link.springer.com/article/10.1007/s11356-022-21154-5 1 27625f46-8690-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=165303 Veterinarska fakulteta 0 0 0