20.500.12556/RUL-143233 Covalently conjugated NOD2/TLR7 agonists are potent and versatile immune potentiators The success of vaccination with subunit vaccines often relies on the careful choice of adjuvants. There is great interest in developing new adjuvants that can elicit a cellular immune response. Here, we address this challenge by taking advantage of the synergistic cross-talk between two pattern recognition receptors: nucleotide-binding oligomerization-domain-containing protein 2 (NOD2) and Toll-like receptor 7 (TLR7). We designed two conjugated NOD2/TLR7 agonists, which showed potent immunostimulatory activities in human primary peripheral blood mononuclear cells and murine bone-marrow-derived dendritic cells. One of these, 4, also generated a strong antigen-specific immune response in vivo, with a Th1-polarized profile. Importantly, our study shows that novel NOD2/TLR7 agonists elicit sophisticated and fine-tuned immune responses that are inaccessible to individual NOD2 and TLR7 agonists. immunostimulatory activity vaccines immune responses pattern recognition receptors synthesis of NOD2 agonist Toll-like receptor 7 imunostimulatorna aktivnost imunski odziv receptorji za prepoznavanje vzorcev sinteza agonista NOD2 receptor TLR7 cepiva true false true Angleški jezik Slovenski jezik Članek v reviji 2022-12-09 07:41:23 2022-12-09 07:41:26 2023-02-16 03:41:11 0000-00-00 00:00:00 2022 0 0 Str. 15085–15101 iss. 22 Vol. 65 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615.371:616-097 1520-4804 10.1021/acs.jmedchem.2c00808 128189187 acs.jmedchem.2c00808.pdf acs.jmedchem.2c00808.pdf 1 8D39BBED6CCD63450262B15C5E707607 2531b68bf6bada9b1c8866891200c1a2b6c4b0a7ac46e4217a3fa990b2adbefe feff6061-ad09-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=166598 https://pubs.acs.org/doi/full/10.1021/acs.jmedchem.2c00808 0 584fd4f2-778c-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=164851 Fakulteta za farmacijo 0 0 0