20.500.12556/RUL-142787 The influence of excipients on the viscosity of monoclonal antibody solutions The aggregation propensity of monoclonal antibodies can be modified by adding different cosolutes into the solution. A simple coarse-grained model in the combination with the thermodynamic perturbation theory was used to predict cluster distribution and viscosity of the solutions of IgG4 monoclonal anibody in the presence of L-Arginine Hydrochloride. The data were analysed using binding polynomial to describe the binding of cosolute (Arginine) to the antibody molecule. The results show that by binding to the antibody molecule the cosolute occupies some of the binding sites of the antibody, and in this way reduces the amount of binding sites available to other antibody molecules. The aggregation propensity of the antibody molecules is therefore reduced. monoclonal antibody aggregation viscosity cosolutes thermodynamic perturbation theory binding polynomial monoklonska protitelesa agregacija viskoznost sotopljenci termodinamska perturbacijska teorija statistična vsota vezanja true false true Angleški jezik Slovenski jezik Članek v reviji 2022-11-25 11:47:53 2022-11-25 11:47:54 2023-01-20 03:37:34 0000-00-00 00:00:00 2022 0 0 5 str. art. 120349 Vol. 366 15 Nov. 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 544.35:577.322 0167-7322 10.1016/j.molliq.2022.120349 121626627 1-s2.0-S0167732222018888-main.pdf 1-s2.0-S0167732222018888-main.pdf 1 1688F7859EF0668E11C48CE1B7B8AC7F 3501e3838b37f98ca3fc495a86defb131c6090fd0e51c42eba6c7b85cf2a5560 e3facc76-6caf-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=164268 https://www.sciencedirect.com/science/article/pii/S0167732222018888 1 7b9f97c9-6cae-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=164267 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0