20.500.12556/RUL-142737 Primerjava parametrov osnovne urinske analize z uporabo dveh analitskih sistemov magistrsko delo Comparison of urinalysis parameters using two analytical systems Avtomatizacija urinskih preiskav postaja vse bolj razširjena praksa v laboratorijski medicini. Z vpeljavo avtomatskih urinskih analizatorjev se poveča količina vzorcev pregledanih v določenem času, zmanjša se potreba po delovni sili in omeji količina napak, ki so posledica človeškega vpliva. Še vedno pa ostaja ročna analiza z mikroskopom “zlati standard”, zato je potrebno prepoznati prednosti in slabosti novih metod preiskovanja. V diplomskem delu testiramo avtomatski urinski analizator AtellicaTM UAS 800, katerega primerjamo z referenčnima metodama – polavtomatskim analizatorjem Cobas u411 in svetlobnim mikroskopom. V delu najprej opišemo proces osnovne urinske analize in podrobneje predstavimo posamične faze ter parametre, ki so v njej analizirani. V nadaljevanju podamo metodološke postopke, uredimo in analiziramo rezultate urinskih vzorcev, ki so bili predhodno analizirani z različnimi analitskimi sistemi. Rezultate prikažemo ločeno za vsako fazo osnovne urinske analize in na podlagi tega dobimo vpogled v ujemanje med uporabljenimi metodami. Ugotovitve kažejo, da je ujemanje med metodama boljše pri fazi kemijske analize s testnimi trakovi, kjer sta primerjana testirani avtomatski in referenčni polavtomatski urinski analizator. Ujemanje med metodama je slabše pri analizi urinskega sedimenta, kjer je testirani analizator primerjan z ročnim mikroskopiranjem. Automation of urine tests is becoming an increasingly widespread practice in laboratory medicine. With the introduction of automatic urine analyzers, the amount of samples examined in a certain time period is increased, the need for manpower is reduced and the amount of human-influenced errors is limited. However, manual analysis with a microscope is still considered the "gold standard", so it is crucial to recognize both the advantages and disadvantages of new urinalysis methods. We test the automatic urine analyzer AtellicaTM UAS 800, which we compare with two reference methods - the semi-automatic analyzer Cobas u411 and a manual microscope. In the paper, we first describe the process of urinalysis and present in more detail the individual phases and the parameters that are analyzed within. Following, we describe used methodology, we arrange and analyze the results of urine samples that were already analyzed with different analytical systems. The results are shown separately for each phase of the urinalysis. Based on this we get an insight into the correspondence between the methods used. The findings show that the agreement between the methods is better in the phase of chemical analysis using test strips, where we compared the tested automatic and reference semi-automatic urine analyzers. The agreement between the methods is poorer in urine sediment analysis, where the tested analyzer is compared with manual microscopy. osnovna urinska analiza avtomatski analizator primerjava metod mikroskopiranje urinalysis automatic analyzer method comparison microscopy true true false [J. Bakija] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2022-11-23 08:30:00 2022-11-23 08:30:08 2022-12-12 07:59:37 0000-00-00 00:00:00 2022 0 Ljubljana 0 Ljubljana 46 str. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 612.461.1:004(043.2) 147270 133275907 10474.pdf 10474.pdf 1 5989DA3C0F96D19E94730CBDD85EF878 3f40f0074ef60fa7e47e97ec82a0d6e084d1bfdeb70102a8c192eb713ae2ccee 8701a530-6b00-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=164209 Fakulteta za družbene vede 0 0 0