20.500.12556/RUL-142358 Antimicrobial resistance and molecular characterization of methicillin-resistant Staphylococcus aureus from two pig farms longitudinal study of LA-MRSA Pigs were identified as the most important reservoir of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA), mostly belonging to the emergent zoonotic clonal complex (CC) 398. Here, we investigated the presence of MRSA in sows and piglets over a period of several months in two pig farms (intensive farm A and family-run farm B). Isolates underwent antimicrobial susceptibility testing, PCR characterization and spa typing. We collected 280 samples, namely 206 nasal swabs from pigs and 74 environmental samples from pig housings at 12 consecutive time points. A total of 120/161 (74.5%) and 75/119 (63.0%) samples were MRSA-positive in farms A and B, respectively. All isolates harbored mecA but lacked mecC and PVL-encoding genes. The identified spa types (t571, t034, t1250 and t898 in farm A, t1451 and t011 in farm B) were indicative of CC398. Antimicrobial resistance patterns (all multidrug resistant in farm A, 57.2% in farm B) depended on the farm, suggesting the impact of farm size and management practices on the prevalence and characteristics of MRSA. Due to the intermittent colonization of pigs and the high contamination of their immediate environment, MRSA status should be determined at the farm level when considering preventive measures or animal trade between farms. livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) pigs multidrug resistance (MDR) spa typing longitudinal study true false true Angleški jezik Ni določen Članek v reviji 2022-11-02 10:57:37 2022-11-02 10:57:38 2022-11-03 03:40:52 0000-00-00 00:00:00 2022 0 0 14 str. no. 11, art. 1532 Vol. 11 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2022-11-02 636.09:579 2079-6382 10.3390/antibiotics11111532 127705091 Antimicrobial-Golob_Majda.pdf Antimicrobial-Golob_Majda.pdf 1 93831FB7E71A690FC6CC75F70FF63BB4 3efefbdcfc3f8edbbdf39fc5bf391ee6e9279b1779f57cb647eff0eaaaa6f51f 7fb18608-5a95-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=163680 https://www.mdpi.com/2079-6382/11/11/1532 1 b30c0fda-5a94-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=163679 Veterinarska fakulteta 0 0 0