20.500.12556/RUL-142154 Application of FTIR spectroscopy to detect changes in skeletal muscle composition due to obesity with insulin resistance and STZ-induced diabetes Age, obesity, and diabetes mellitus are pathophysiologically interconnected factors that significantly contribute to the global burden of non-communicable diseases. These metabolic conditions are associated with impaired insulin function, which disrupts the metabolism of carbohydrates, lipids, and proteins and can lead to structural and functional changes in skeletal muscle. Therefore, the alterations in the macromolecular composition of skeletal muscle may provide an indication of the underlying mechanisms of insulin-related disorders. The aim of this study was to investigate the potential of Fourier transform infrared (FTIR) spectroscopy to reveal the changes in macromolecular composition in weight-bearing and non-weight-bearing muscles of old, obese, insulin-resistant, and young streptozotocin (STZ)-induced diabetic mice. The efficiency of FTIR spectroscopy was evaluated by comparison with the results of gold-standard histochemical techniques. The differences in biomolecular phenotypes and the alterations in muscle composition in relation to their functional properties observed from FTIR spectra suggest that FTIR spectroscopy can detect most of the changes observed in muscle tissue by histochemical analyses and more. Therefore, it could be used as an effective alternative because it allows for the complete characterization of macromolecular composition in a single, relatively simple experiment, avoiding some obvious drawbacks of histochemical methods. Fourier transform infrared (FTIR) spectroscopy histochemical analysis insulin resistance obesity diabetes skeletal muscle macromolecular composition medicina diabetes debelost mišji modeli skeletne mišice makromolekularna sestava spektroskopija FTIR true false true Angleški jezik Slovenski jezik Članek v reviji 2022-10-21 13:17:03 2022-10-21 13:17:04 2022-10-22 03:57:55 0000-00-00 00:00:00 2022 0 0 22 str. no. 20 Vol. 23 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616 1422-0067 10.3390/ijms232012498 126210563 ijms-23-12498-v2.pdf ijms-23-12498-v2.pdf 1 5C456A015C9035E332F6ED2574CFA22F c8bb55494abd50d3d8f28f241781cdcb2861cd49d6b61953845b256c6c0adbe2 08e2a3cd-5133-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=163436 https://www.mdpi.com/1422-0067/23/20/12498 1 d5532ec4-5131-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=163435 Medicinska fakulteta Biotehniška fakulteta 0 0 0