20.500.12556/RUL-142110 First detection of honeybee pathogenic viruses in butterflies Several pathogens are important causes of the observed pollinator decline, some of which could be transmitted between different pollinator species. To determine whether honeybee viruses can be transmitted to butterflies, a total of 120 butterflies were sampled at four locations in Slovenia. At each location, butterflies from three families (Pieridae, Nymphalidae, Hesperiidae/Lycenidae) and Carniolan honeybees (Apis mellifera carnica) were collected. The RNA of six honeybee viruses, i.e., acute bee paralysis virus (ABPV), black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), deformed wing virus A (DWV-A), Sacbrood bee virus (SBV), and Lake Sinai virus 3 (LSV3), was detected by a specific quantitative method (RT-PCR). The presence of ABPV, BQCV, LSV3, and SBV was detected in both butterflies and honeybees. All butterfly and bee samples were negative for CBPV, while DWV-A was detected only in honeybees. The viral load in the positive butterfly samples was much lower than in the positive bee samples, which could indicate that butterflies are passive carriers of bee viruses. The percentage of positive butterfly samples was higher when the butterflies were collected at sampling sites with a higher density of apiaries. Therefore, we believe that infected bees are a necessary condition for the presence of viruses in cohabiting butterflies. This is the first study on the presence of pathogenic bee viruses in butterflies. butterflies honeybees viruses pathogens transmission bees virology true false true Angleški jezik Ni določen Članek v reviji 2022-10-20 14:25:16 2022-10-20 14:25:17 2024-02-28 03:47:54 0000-00-00 00:00:00 2022 0 0 12 str. iss. 10, art. 925 Vol. 13 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 578 2075-4450 10.3390/insects13100925 126058243 insects-13-00925-v2.pdf insects-13-00925-v2.pdf 1 D9EF05044CD497AC0737DAF4AF9751DD 6c8f49a84fddc4bdbc3a39aadcd04f9e04f1ac3be3c9412bcf43abb768f492a3 3f9a1f2c-d55b-11ee-9672-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=181285 https://www.mdpi.com/2075-4450/13/10/925 0 5643bc7c-d55b-11ee-9672-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=181286 Veterinarska fakulteta Biotehniška fakulteta 0 0 0