20.500.12556/RUL-141863 Tailoring copper(II) complexes with pyridine-4,5-dicarboxylate esters for anti-Candida activity Five novel copper(II) complexes with pyridine-4,5-dicarboxylate esters as ligands, [Cu(NO$_3$)(py-2tz)(H$_2$O)$_3$]NO$_3$ (1), [Cu(NO$_3$)$_2$(py-2metz)(H$_2$O)] (2), [Cu(NO$_3$)$_2$(py-2py)(H$_2$O)]·H$_2$O (3), [CuCl$_2$(py-2tz)]$_2$ (4) and [CuCl$_2$(py-2metz)]$_n$ (5) (py-2tz is dimethyl 2-(thiazol-2-yl)pyridine-4,5-dicarboxylate, py-2metz is dimethyl 2-(4-methylthiazol-2-yl)pyridine-4,5-dicarboxylate and py-2py is dimethyl 2,2’-bipyridine-4,5-dicarboxylate), were synthesized and structurally characterized by different spectroscopic and electrochemical methods. The structure of these complexes was determined by single-crystal X-ray diffraction analysis, confirming the bidentate coordination mode of the corresponding pyridine-4,5-dicarboxylate ester to the Cu(II) ion through the nitrogen atoms. The antimicrobial potential of copper(II) complexes 1–5 was assessed against two bacterial and two Candida species. These complexes showed better growth inhibiting activity against Candida spp. with respect to the tested bacterial species, also being moderately toxic towards normal human lung fibroblast cells (MRC-5). Complexes 1 and 4 showed the greatest ability to inhibit the filamentation of C. albicans, which is an important process during fungal infection, and these two complexes efficiently inhibited the biofilm formation of C. albicans at subinhibitory concentrations. Complex 4 also successfully prevented the adhesion of C. albicans in an in vitro epithelial cell model. The mechanism of the antifungal activity of copper(II) complexes 1–5 was studied through their interaction with ct-DNA, as one of the possible target biomolecules, by fluorescence spectroscopy and gel electrophoresis. Finally, the ability of these complexes to bind to bovine serum albumin (BSA) was studied by fluorescence emission spectroscopy. copper(II) complexes pyridine-4 5-dicarboxylates antimicrobial activity Candida albicans filamentation DNA/BSA interaction bakrovi(II) kompleksi piridin-4 5-dikarboksilati protimikrobna aktivnost Candida albicans filamentacija DNA/BSA interakcija true false true Angleški jezik Slovenski jezik Članek v reviji 2022-10-10 09:15:43 2022-10-10 09:15:45 2022-11-08 03:56:52 0000-00-00 00:00:00 2021 0 0 Str. 2627-2638 iss. 7 Vol. 50 21 Feb. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 546.562:547.82 1477-9234 10.1039/D0DT04061D 50313731 d0dt04061d.pdf d0dt04061d.pdf 1 3DF85F6D69E4F8619362FBF1CA38DF5E 6abd9d8e25995ccfb904f4c98ba8f3bb04c364d33ba0aee223db2c3b3ef479af a7ab528b-5ea6-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=163780 https://pubs.rsc.org/en/content/articlelanding/2021/dt/d0dt04061d 0 53a4bc9c-486b-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=162996 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0