20.500.12556/RUL-141254 Enrichment of plasma in platelets and extracellular vesicles by the counterflow to erythrocyte settling In order to prepare optimal platelet and extracellular vesicle (EV)-rich plasma for the treatment of chronic temporal bone inflammation, we studied effects of centrifugation parameters on redistribution of blood constituents in blood samples of 23 patients and 20 volunteers with no record of disease. Concentrations of blood cells and EVs were measured by flow cytometry. Sample content was inspected by scanning electron microscopy. A mathematical model was constructed to interpret the experimental results. The observed enrichment of plasma in platelets and EVs after a single spin of blood depended on the erythrocyte sedimentation rate, thereby indicating the presence of a flow of plasma that carried platelets and EVs in the direction opposite to settling of erythrocytes. Prolonged handling time correlated with the decrease of concentration of platelets and larger EVs in platelet and EV-rich plasma (PVRP), R = −0.538, p = 0.003, indicating cell fragmentation during the processing of samples. In further centrifugation of the obtained plasma, platelet and EV enrichment depended on the average distance of the sample from the centrifuge rotor axis. Based on the agreement of the model predictions with observations, we propose the centrifugation protocol optimal for platelet and EV enrichment and recovery in an individual sample, adjusted to the dimensions of the centrifuge rotor, volume of blood and erythrocyte sedimentation rate. cellular vesicles extracellular vesicles microvesicles platelet microparticles platelet rich plasma wound healing true false true Angleški jezik Ni določen Članek v reviji 2022-09-27 08:21:49 2022-09-27 08:21:50 2022-09-28 04:04:29 0000-00-00 00:00:00 2022 0 0 Str. 592-602 iss. 4 Vol. 33 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616 0953-7104 10.1080/09537104.2021.1961716 74092547 Enrichment_of_plasma_in_platelets_and_extracellular_vesicles_by_the_counterflow_to_erythrocyte_settling.pdf Enrichment_of_plasma_in_platelets_and_extracellular_vesicles_by_the_counterflow_to_erythrocyte_settling.pdf 1 18DA05A036B4D91BB7396D0C3A2E1B58 b49565000011217bc7efed9f39b89ffd12ffdb5f3201f4ebb3cee4f8ed34d1d4 01236268-3e2d-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=162222 https://www.tandfonline.com/doi/full/10.1080/09537104.2021.1961716 0 d66ab0aa-3e2c-11ed-9c94-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=162221 Zdravstvena fakulteta Fakulteta za elektrotehniko Medicinska fakulteta 0 0 0