20.500.12556/RUL-141065 Vpliv krme z večjo vsebnostjo maščobe na sestavo telesa pri mišjih linijah FLI in FHI Effects of high fat diet content on body composition in FLI and FHI mouse lines Cilj naloge je bil zbrati in analizirati podatke iz postopka krmljenja vitke linje miši (FHI) in debele linije miši (FLI). Liniji sta bili krmljeni z krmama, kjer ima ena večjo vsebnost maščob kot druga. Zanimalo nas je ali imata ta dva tipa krme vpliv na diferencialno prirast miši in maščobnih depojev v odvisnosti od genotipa. Debelost je povezana z rezistenco na inzulin. Raziskave kažejo, da imajo maščobna tkiva pomembno vlogo pri nastanku in napredovanju bolezni povezanih z debelostjo. Visok vnos maščob vodi do sprememb v presnovi v maščobnih tkivih, te pa povečajo raven prostih maščobnih kislin v krvi. V postopku smo uporabili 42 miši linij FHI in FLI (24 FHI, 18 FLI). Miši so bile vključene v postopek pri starosti 21-25 dni. Miši so bile ločene v dve skupini pri vsaki liniji in so dobivale dve vrsti krme. Ena skupina je dobivala krmo z nizko vsebnostjo maščobe (LFD). Druga skupina pa je dobivala krmo z visoko vsebnostjo maščob (HFD). Upoštevajoč dobljene rezultate, lahko sklepamo, da se genotipa v merjenih lastnostih nista razlikovala pri krmi LFD. Pri skupini, ki je bila krmljena z HFD krmo, pa je vpliv genotipa statistično značilen. The aim of this study was to collect and analyze data from an experiment in which we fed isocaloric diets to a lean line (FHI) and a fat line (FLI) of mice, but with different compositions-a high fat diet (HFD) and a low fat diet (LFD). We were interested in whether these two types of diets had an effect on the difference in growth of the mice and fat deposition depending on the genotype FHI or FLI. Obesity is associated with insulin resistance. Research shows that adipose tissue plays an important role in the development and progression of diseases related to obesity. High fat intake leads to changes in adipose tissue metabolism, increasing the level of free fatty acids in the blood, which has a negative effect on metabolism. We used 42 mice of the FHI and FLI lines (24 FHI, 18 FLI) for the procedure. The mice were enrolled in the study at 21-25 days of age. They were divided into two groups for each line. One group was fed low-fat diet and the other high fat diet. The results showed that the effect of genotype was not significant in the low-fat treatment. In the group fed high fat diet, we found statistically significant differences in the measured traits between FLI and FHI genotypes. prehrana živali maščobe laboratorijske miši linije sestava telesa animal nutrition fat diet laboratory mice lines body composition true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2022-09-23 07:15:24 2022-09-23 07:15:35 2022-09-27 08:24:23 0000-00-00 00:00:00 2022 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 211929 123005187 29398.pdf 29398.pdf 1 6855BEE67E9C02FB3DAAB926CFF2DDEF c58cf75e8254123d572049cfcd52d0a417b84dfc3a169e294e4e33ec84729bc9 ac8af3ec-3afe-11ed-92af-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=161961 Biotehniška fakulteta 0 0 0