20.500.12556/RUL-140823 Spatial distribution and stability of cholinesterase inhibitory protoberberine alkaloids from Papaver setiferum During a research program to identify new cholinesterase inhibitors of natural origin, two new 7,8-didehydroprotoberberine alkaloids (1 and 2) and nine known compounds (3−11) were isolated from the capsules of the common ornamental poppy, Papaver setiferum (previously P. pseudo-orientale). Despite their reported instability, the 7,8-didehydroprotoberberines isolated herein appeared relatively stable, particularly as their trifluoroacetic acid salts. The spatial distributions of the isolated alkaloids were also analyzed using desorption electrospray ionization imaging mass spectrometry. The alkaloids were localized predominantly within the walls and vascular bundles of the capsules, with the highest relative abundances occurring in the lower half of the capsules toward the peduncle. The relative abundances of the alkaloids were also compared across plant development stages. Although most alkaloids did not show clear patterns in their concentration across development stages, the concentration of suspected oxidation products clearly spiked upon plant death. Finally, all isolated natural products were screened for inhibitory activities against a panel of cholinesterases, from both human and animal sources. These studies identified several competitive inhibitors of cholinesterases with potency in the low micromolar range (1−4, 6, 7), offering new lead compounds for the development of cholinesterase inhibitory drugs Cholinesterase Inhibitors Papaver Alkaloids true false true Angleški jezik Ni določen Neznano 2022-09-19 09:02:28 2022-09-19 09:02:28 2022-09-20 04:04:43 0000-00-00 00:00:00 2022 0 0 Str. 215-224 no. 1 Vol. 85 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2022-01-01 577 1520-6025 10.1021/acs.jnatprod.1c00980 89689603 spatial-trobec.pdf spatial-trobec.pdf 1 9FDC39EC268FD2B0551E73995350AE6A f6c6aa27a444376a2c4515e74df30cc260ffe2eda7610bfafdf50cb2fd2decf9 8cfc4e27-37ec-11ed-92af-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=161624 https://pubs.acs.org/doi/pdf/10.1021/acs.jnatprod.1c00980 1 f2ae5c6c-37e8-11ed-92af-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=161623 Veterinarska fakulteta Biotehniška fakulteta 0 0 0