20.500.12556/RUL-140238 In vitro characterization of the human skeletal stem cell-like properties of primary bone-derived mesenchymal stem /stromal cells in patients with late and early hip osteoarthritis Human skeletal stem cells (hSSCs) were recently identified as podoplanin (PDPN)/CD73/CD164-positive and CD146-negative cells that decline with age, and play a role in the pathogenesis of osteoarthritis (OA). The aim of this study was to identify the hSSC-like properties of bone-derived mesenchymal stem/stromal cells (MSCs) of patients with late and early OA. Methods: First, we performed gene expression profiling for the hSSC markers in 32 patients with late and early OA, and donors without OA. Having identified the low expression of hSSC markers in late OA patients, we further performed trilineage differentiation and immunophenotyping for hSSC makers in the selected subsets from each donor group. Results: Our results show no differences in osteogenesis, chondrogenesis, and adipogenesis between the MSCs from the three groups. However, the immunophenotyping shows lower CD164 in MSCs from early OA patients in comparison with late and no OA subjects (p = 0.002 and p = 0.017). Conclusions: Our study shows that the in vitro hSSC-like properties of bone-derived MSCs are similar in patients with early and late OA, and in donors without OA. However, the lower percentage of CD164-positive MSCs in early OA patients indicates the potential of CD164 as a marker of the onset of OA. human skeletal stem cells (hSSCs) late osteoarthritis (OA) early OA bone-derived mesenchymal stem/stromal cells (MSCs) podoplanin (PDPN) CD73 CD164 CD146 trilineage differentiation immunophenotyping artroza kostne matične celice kolk človeške skeletne izvorne celice (hSSC) pozni osteoartritis (OA) zgodnji OA mezenhimske matične/stromalne celice (MSC) iz kosti podoplanin (PDPN) CD73 CD164 CD146 trirodnost diferenciacija imunofenotipizacija true false true Angleški jezik Slovenski jezik Članek v reviji 2022-09-13 13:17:10 2022-09-13 13:17:13 2022-09-16 04:13:56 0000-00-00 00:00:00 2022 0 0 Str. 1-15 iss. 6, art. 899 Vol. 12 2022 0000-00-00 Zaloznikova NiDoloceno NiDoloceno 0000-00-00 0000-00-00 2022-01-01 617.3 2075-1729 10.3390/life12060899 112107267 life-12-00899-v3.pdf life-12-00899-v3.pdf 1 37490B7D70B20D6149AA2F3B5D526C17 c05fa3f32a22d302fb572867a2ca56697ec58c70839d01e2c17da75a70ce7f9f 192d269b-3356-11ed-92af-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=160934 https://www.mdpi.com/2075-1729/12/6/899 1 8f034816-3355-11ed-92af-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=160933 Fakulteta za farmacijo Medicinska fakulteta 0 0 0