20.500.12556/RUL-139485 Raziskave odpornosti rastlin na okužbe z viroidi na molekularni ravni Researches on plant resistance to viroid infections at the molecular level Viroidi so skupina enoverižnih kovalentno zaprtih RNA molekul, ki jih delimo v družini Pospiviroidae in Avsunviroidae. So najmanjši rastlinski patogeni, ki na različne načine povzročijo bolezensko stanje pri gostiteljskih rastlinah (krompir, paradižnik, hmelj, vinska trta, agrumi …). Patogeneza je lahko sprožena prek interakcije med zrelo viroidno RNA molekulo in rastlinskimi proteini (strukturno specifično) ali prek malih viroidnih inferenčnih RNA molekul, ki nastanejo ob DCL procesiranju (specifično glede na nukleotidno zaporedje). Ob razumevanju delovanja viroidov so bile razvite metode za izdelavo tolerantnih rastlin na okužbo z viroidom. Največji potencial predstavljajo metode transgenze, kjer lahko z vnosom lasničnih RNA ali umetnih malih RNA preko aktivacije RNA interference zaščitimo rastline. Viroids are a group of single-stranded covalently closed RNA molecules, which belong in two families Pospiviroidae and Avsunviroidae. They are the smallest plant pathogens, that can elicit disease state at host plants (potato, tomato, hops, vine, citruses …). Pathogenesis can be triggered with interaction between the mature RNA molecule and plant proteins (structurally specific) or via viroid small interfering RNA molecules, which are produced in DCL processing (nucleotide sequence specific). Understanding how viroids work, methods for viroid-tolerant plants are being developed. The greatest potential is presented by transgenesis approaches, where plants can be protected by insertion of hairpin RNA or artificial small RNA through the activation of RNA interference. Viroid rastline RNA molekula patogenost male RNA metilacija utišanje genov rezistenca metode transgeneze ribonukleaza katalitično protitelo RNA polimeraza protismiselna RNA »hammerhead« ribocim lasnična RNA umetne mikroRNA Viroid plants RNA molecule pathogenesis small RNA methylation gene silencing resistence transgenic approaches ribonucleases catalytic antibody RNA polymerase antisense RNA hammerhead ribozyme hairpin RNA artificial miRNA true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2022-09-03 07:15:12 2022-09-03 07:16:09 2022-10-10 14:55:52 0000-00-00 00:00:00 2022 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 208452 124982275 24728.pdf 24728.pdf 1 82575A3A30EDD546CE1EF2BFF57A7B7C 04730ff61c86d7ac0f5d6245f16097c70d37bd048335508ec0b3900ce443bb0a 7fd1ffe6-2b47-11ed-92af-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=160055 Biotehniška fakulteta 0 0 0