20.500.12556/RUL-139099 Cell models for Alzheimer’s and Parkinson’s disease at the interface of biology and drug discovery Neurodegenerative diseases are severely debilitating conditions characterized primarily by progressive neuronal loss and impairment of the nervous system. Alzheimer’s and Parkinson’s diseases are the most common neurodegenerative disorders, and their impact is increasing as average life expectancy increases worldwide. Although the underlying mechanisms of both progressive diseases have been extensively studied, we still lack a comprehensive understanding of the molecular basis of both diseases. Current therapeutic options do not slow the progression of the diseases and only provide symptom relief. Cell models that resemble the characteristics of the disease in question are important in drug discovery projects because they provide information about the therapeutic benefits of drugs under development. Here, we review current in vitro cell models used to study the molecular basis of Alzheimer’s and Parkinson’s disease focusing on their potential for discovering of disease-modifying therapeutics to combat neurodegenerative diseases. We discuss phenotypic screening as an important approach for identifying novel therapeutic molecules. Advances in the development of cell-based assays for drug discovery are discussed, ranging from simple monoculture cell models to high-throughput three-dimensional cell models. Finally, we critically present the limitations of cell models and the caveats encountered in drug discovery to find effective treatment for neurodegenerative diseases. neurodegenerative diseases cell models cytotoxicity assays drug discovery drug screening nevrodegenerativne bolezni celični modeli preskusi citotoksičnosti odkrivanje zdravil Alzheimerjeva bolezen Parkinsonova bolezen farmacevtska kemija true false true Angleški jezik Slovenski jezik Članek v reviji 2022-08-30 16:04:13 2022-08-30 16:04:17 2022-09-15 04:17:51 0000-00-00 00:00:00 2022 0 0 11 str. Vol. 149, art. 112924 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615.4:54:616.831-003.8 1950-6007 10.1016/j.biopha.2022.112924 105487619 1-s2.0-S0753332222003134-main_(1).pdf 1-s2.0-S0753332222003134-main_(1).pdf 1 86A0A1CC400C802BB1FF16A54A9D8C08 867bf5a1b02c7d5ff7371148b3cb48d0d5eaa9643d897bedb8742efba904ca32 fde599ec-286c-11ed-92af-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=159635 https://www.sciencedirect.com/science/article/pii/S0753332222003134 1 9a511899-286c-11ed-92af-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=159634 Fakulteta za farmacijo 0 0 0