20.500.12556/RUL-139090 Redox active or thiol reactive? optimization of rapid screens to identify less evident nuisance compounds Compounds that exhibit assay interference or undesirable mechanisms of bioactivity are routinely encountered in assays at various stages of drug discovery. We observed that assays for the investigation of thiol-reactive and redox-active compounds have not been collected in a comprehensive review. Here, we review these assays and subject them to experimental optimization to improve their reliability. We demonstrate the usefulness of our assay cascade by assaying a library of bioactive compounds, chemical probes, and a set of approved drugs. These high-throughput assays should complement the array of wet-lab and in silico assays during the initial stages of hit discovery campaigns to pursue only hit compounds with tractable mechanisms of action. redox covalent screening hits PAINS assays redoks kovalentni presejalni pregled testi farmacevtska kemija zdravila true false true Angleški jezik Slovenski jezik Članek v reviji 2022-08-30 13:06:04 2022-08-30 13:06:08 2022-09-15 04:17:50 0000-00-00 00:00:00 2022 0 0 Str. 1733-1742 no. 6 Vol. 27 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615.4:54 1359-6446 10.1016/j.drudis.2022.03.008 100881923 1-s2.0-S1359644622001167-main_(1).pdf 1-s2.0-S1359644622001167-main_(1).pdf 1 337A6DD3A8F2FD886FBFFDB28A3C2878 97e5bc0d226b15bd949ed57cba17586c4a67ef6aefca1c6a9dc6c17c3555a173 1f0eee94-2854-11ed-92af-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=159625 https://www.sciencedirect.com/science/article/pii/S1359644622001167 1 b731174c-2853-11ed-92af-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=159622 Fakulteta za farmacijo 0 0 0