20.500.12556/RUL-138924 Salivary gland adaptation to dietary inclusion of hydrolysable tannins in boars The ingestion of hydrolysable tannins as a potential nutrient to reduce boar odor in entire males results in the significant enlargement of parotid glands (parotidomegaly). The objective of this study was to characterize the effects of different levels of hydrolysable tannins in the diet of fattening boars (n = 24) on salivary gland morphology and proline-rich protein (PRP) expression at the histological level. Four treatment groups of pigs (n = 6 per group) were fed either a control (T0) or experimental diet, where the T0 diet was supplemented with 1% (T1), 2% (T2), or 3% (T3) of the hydrolysable tannin-rich extract Farmatan$^®$. After slaughter, the parotid and mandibular glands of the experimental pigs were harvested and dissected for staining using Goldner’s Trichrome method, and immunohistochemical studies with antibodies against PRPs. Morphometric analysis was performed on microtome sections of both salivary glands, to measure the acinar area, the lobular area, the area of the secretory ductal cells, and the sizes of glandular cells and their nuclei. Histological assessment revealed that significant parotidomegaly was only present in the T3 group, based on the presence of larger glandular lobules, acinar areas, and their higher nucleus to cytoplasm ratio. The immunohistochemical method, supported by color intensity measurements, indicated significant increases in basic PRPs (PRB2) in the T3 and acidic PRPs (PRH1/2) in the T1 groups. Tannin supplementation did not affect the histo-morphological properties of the mandibular gland. This study confirms that pigs can adapt to a tannin-rich diet by making structural changes in their parotid salivary gland, indicating its higher functional activity. pigs dietary supplements tannins parotid gland mandibular gland immunohistochemistry histology proline-rich proteins PRP swine true false true Angleški jezik Ni določen Članek v reviji 2022-08-25 11:20:48 2022-08-25 11:20:49 2023-05-16 03:32:37 0000-00-00 00:00:00 2022 0 0 14 str. iss. 17, art. 2171 Vol. 12 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09:591 2076-2615 10.3390/ani12172171 119067139 animals-12-02171-v2.pdf animals-12-02171-v2.pdf 1 A2FB48DE72E21696350DDE3BA8034CE7 4a1dcaf32ea176abaf38bc8275634761f5ea578b46752176775835c1fba03631 528783c9-f2ef-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168804 https://www.mdpi.com/2076-2615/12/17/2171 0 2fbdc20f-2457-11ed-92af-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=159421 Veterinarska fakulteta 0 0 0