20.500.12556/RUL-138699 Seroprevalence of severe acute respiratory syndrome coronavirus 2 in Slovenia results of two rounds of a nationwide population study on a probability-based sample, challenges and lessons learned Objectives: Seroprevalence surveys provide crucial information on cumulative severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exposure. This Slovenian nationwide population study is the first longitudinal 6-month serosurvey using probability-based samples across all age categories. Methods: Each participant supplied two blood samples: 1316 samples in April 2020 (first round) and 1211 in October/November 2020 (second round). The first-round sera were tested using Euroimmun Anti-SARS-CoV-2 ELISA IgG (ELISA) and, because of uncertain estimates, were retested using Elecsys Anti-SARS-CoV-2 (Elecsys-N) and Elecsys Anti-SARS-CoV-2 S (Elecsys-S). The second-round sera were concomitantly tested using Elecsys-N/Elecsys-S. Results: The populations of both rounds matched the overall population (n = 3000), with minor settlement type and age differences. The first-round seroprevalence corrected for the ELISA manufacturer's specificity was 2.78% (95% highest density interval [HDI] 1.81%–3.80%), corrected using pooled ELISA specificity calculated from published data 0.93% (95% CI 0.00%–2.65%), and based on Elecsys-N/Elecsys-S results 0.87% (95% HDI 0.40%–1.38%). The second-round unadjusted lower limit of seroprevalence on 11 November 2020 was 4.06% (95% HDI 2.97%–5.16%) and on 3 October 2020, unadjusted upper limit was 4.29% (95% HDI 3.18%–5.47%). Conclusions: SARS-CoV-2 seroprevalence in Slovenia increased four-fold from late April to October/ November 2020, mainly due to a devastating second wave. Significant logistic/methodological challenges accompanied both rounds. The main lessons learned were a need for caution when relying on manufacturer-generated assay evaluation data, the importance of multiple manufacturer-independent assay performance assessments, the need for concomitant use of highly-specific serological assays targeting different SARS-CoV-2 proteins in serosurveys conducted in low-prevalence settings or during epidemic exponential growth and the usefulness of a Bayesian approach for overcoming complex methodological challenges. coronavirus disease 2019 probability-based sample severe acute respiratory syndrome seroprevalence severe acute respiratory syndrome coronavirus 2 koronavirusna bolezen 2019 vzorec na podlagi verjetnosti hud akutni respiratorni sindrom true false true Angleški jezik Slovenski jezik Članek v reviji 2022-08-10 08:25:51 2022-08-10 08:26:04 2022-09-15 04:17:04 0000-00-00 00:00:00 2021 0 0 Str. 1039.e1-1039.e7 iss. 7 Vol. 27 Jul. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.9 1198-743X 10.1016/j.cmi.2021.03.009 62248195 1-s2.0-S1198743X21001440-main.pdf 1-s2.0-S1198743X21001440-main.pdf 1 4EB7CD8A510CB31E1366A277B90CFE6F 015ae0eb2a817992fc3a74ce9283241d3adf16d5f0c5b8269598164ad7d079b8 06bcf63d-1876-11ed-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=159111 https://www.sciencedirect.com/science/article/pii/S1198743X21001440 0 067297ee-1875-11ed-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=159108 Medicinska fakulteta Fakulteta za računalništvo in informatiko Fakulteta za družbene vede 0 0 0