20.500.12556/RUL-138667 Contactless delivery of plasmid encoding EGFP in vivo by High-Intensity Pulsed Electromagnetic Field High-Intensity Pulsed Electromagnetic Fields (HI-PEMF) treatment is an emerging noninvasive and contactless alternative to conventional electroporation, since the electric field inside the tissue is induced remotely by external pulsed magnetic field. Recently, HI-PEMF was applied for delivering siRNA molecules to silence enhanced green fluorescent protein (EGFP) in tumors in vivo. Still, delivered siRNA molecules were 21 base pairs long, which is 200-times smaller compared to nucleic acids such as plasmid DNA (pDNA) that are delivered in gene therapies to various targets to generate therapeutic effect. In our study, we demonstrate the use HI-PEMF treatment as a feasible noninvasive approach to achieve in vivo transfection by enabling the transport of larger molecules such as pDNA encoding EGFP into muscle and skin. We obtained a long-term expression of EGFP in the muscle and skin after HI-PEMF, in some mice even up to 230 days and up to 190 days, respectively. Histological analysis showed significantly less infiltration of inflammatory mononuclear cells in muscle tissue after the delivery of pEGFP using HI-PEMF compared to conventional gene electrotransfer. Furthermore, the antitumor effectiveness using HI-PEMF for electrotransfer of therapeutic plasmid, i.e., silencing MCAM was demonstrated. In conclusion, feasibility of HI-PEMF was demonstrated for transfection of different tissues (muscle, skin, tumor) and could have great potential in gene therapy and in DNA vaccination. magnetic fields electric fields membrane permeabilization electroporation enhanced green fluorescent protein mouse melanoma model magnetno polje električno polje permeabilizacija membrane elektroporacija zeleni fluorescenčni protein true false true Angleški jezik Slovenski jezik Članek v reviji 2022-08-08 14:16:40 2022-08-08 14:16:42 2022-09-15 04:17:02 0000-00-00 00:00:00 2021 0 0 10 str. art. 107847 Vol. 141 Oct. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602.621 1567-5394 10.1016/j.bioelechem.2021.107847 64622083 1-s2.0-S1567539421001109-main.pdf 1-s2.0-S1567539421001109-main.pdf 1 0261BD011747A8041F0660208588B823 242f9d7b270945fa2d65de3f34f3b9e6327289dd4f90dae094f08a33ef80489b ff8907eb-1713-11ed-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=159053 https://www.sciencedirect.com/science/article/pii/S1567539421001109 0 ad2a7fb9-1713-11ed-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=159052 Fakulteta za elektrotehniko Medicinska fakulteta Zdravstvena fakulteta 0 0 0