20.500.12556/RUL-138666 Electroporation with nanosecond pulses and bleomycin or cisplatin results in efficient cell kill and low metal release from electrodes Nanosecond electric pulses have several potential advantages in electroporation-based procedures over the conventional micro- and millisecond pulses including low level of heating, reduced electrochemical reactions and reduced muscle contractions making them alluring for use in biomedicine and food industry. The aim of this study was to evaluate if nanosecond pulses can enhance the cytotoxicity of chemotherapeutics bleomycin and cisplatin in vitro and to quantify metal release from electrodes in comparison to 100 μs pulses commonly used in electrochemotherapy. The effects of nanosecond pulse parameters (voltage, pulse duration, number of pulses) on cell membrane permeabilization, resealing and on cell survival after electroporation only and after electrochemotherapy with bleomycin and cisplatin were evaluated on Chinese hamster ovary cells. Application of permeabilizing nanosecond pulses in combination with chemotherapeutics resulted in successful cell kill. Higher extracellular concentrations of bleomycin – but not cisplatin – were needed to achieve the same decrease in cell survival with nanosecond pulses as with eight 100 μs pulses, however, the tested bleomycin concentrations were still considerably lower compared to doses used in clinical practice. Decreasing the pulse duration from microseconds to nanoseconds and concomitantly increasing the amplitude to achieve the same biological effect resulted in reduced release of aluminum ions from electroporation cuvettes. electroporation nanosecond pulses electrochemotherapy metal release elektroporacija nanosekundni pulzi elektrokemoterapija sproščanje kovin true false true Angleški jezik Slovenski jezik Članek v reviji 2022-08-08 13:35:59 2022-08-08 13:36:01 2022-09-15 04:17:02 0000-00-00 00:00:00 2021 0 0 12 str. art. 107798 Vol. 140 Aug. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602.621:616-006 1567-5394 10.1016/j.bioelechem.2021.107798 57144835 1-s2.0-S156753942100061X-main.pdf 1-s2.0-S156753942100061X-main.pdf 1 895C17A0D2D4E9B59D16FA33A8748B79 3cf78649af186b9944d107f98650cf101722fbd5e8a69856222d75bf39ed9e31 6c4b8e5a-170e-11ed-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=159051 https://www.sciencedirect.com/science/article/pii/S156753942100061X 0 2ea05413-170e-11ed-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=159050 Fakulteta za elektrotehniko 0 0 0