20.500.12556/RUL-138300 Development of microsatellite markers for Tanacetum cinerariifolium (Trevis.) Sch. Bip., a plant with a large and highly repetitive genome Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevis.) Sch. Bip.) is an outcrossing plant species (2n = 18) endemic to the eastern Adriatic coast and source of the natural insecticide pyrethrin. Due to the high repeatability and large genome (1C-value = 9.58 pg) our previous attempts to develop microsatellite markers using the traditional method were unsuccessful. Now we have used Illumina paired-end whole genome sequencing and developed a specific procedure to obtain useful microsatellite markers. A total of 796,130,142 high-quality reads (approx. 12.5× coverage) were assembled into 6,909,675 contigs using two approaches (de novo assembly and joining of overlapped pair-end reads). A total of 31,380 contigs contained one or more microsatellite sequences, of which di-(59.7%) and trinucleotide (25.9%) repeats were the most abundant. Contigs containing microsatellites were filtered according to various criteria to achieve better yield of functional markers. After two rounds of testing, 17 microsatellite markers were developed and characterized in one natural population. Twelve loci were selected for preliminary genetic diversity analysis of three natural populations. Neighbor-joining tree, based on the proportion of shared alleles distances, grouped individuals into clusters according to population affiliation. The availability of codominant SSR markers will allow analysis of genetic diversity and structure of natural Dalmatian pyrethrum populations as well as identification of breeding lines and cultivars. Tanacetum cinerariifolium Dalmatian pyrethrum Asteraceae genomic SSRs microsatellite markers SSR markers dalmatinski bolhač genetika mikrosatelitski markerji genetska raznolikost SSR markerji true false true Angleški jezik Slovenski jezik Članek v reviji 2022-07-14 09:31:18 2022-07-14 09:31:21 2022-09-15 04:16:31 0000-00-00 00:00:00 2022 0 0 11 str. iss. 13, art. 1778 Vol. 11 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 633 2223-7747 10.3390/plants11131778 115302403 plants-11-01778.pdf plants-11-01778.pdf 1 1B371CF039C6D4B546EDF27C2A40BD7F 231c4b404f1fe5941105a696ed05a90963adb5c024f9963ed2bcb2cbc4f4a165 fea374c4-0347-11ed-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=158582 https://www.mdpi.com/2223-7747/11/13/1778 1 ba1c73b7-0346-11ed-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=158575 Biotehniška fakulteta 0 0 0