20.500.12556/RUL-138145 Microfluidics-based preparation and in situ immobilization of nanoscale cross-linked enzyme aggregates for continuous transamination research data underlying the article A new microfluidics-based method for the generation and further continuous use of amine transaminase cross-linked enzyme aggregates (ATA-CLEAs) has been developed. The precipitation and cross-linking are carried out in separate but interconnected parts, which allows optimization of each step and thus better final quality of the preparation. After optimizing the acetone and glutaraldehyde concentrations for precipitation and crosslinking, respectively, ATA-CLEAs prepared in a microfluidic device exhibited 87.1% recovered activities, which is 2.4-fold higher than the values previously reported for ATA-CLEAs. The prepared ATA-CLEAs with an average particle radius of 37.13 ± 0.38 nm showed also higher activity than the free enzyme at 70°C. Further integration of a membrane microreactor in the system enabled in situ ATA-CLEA immobilization with 100% yield and up to 68.4% efficiency. The immobilization efficiency and operational stability of the membrane reactor with ATA-CLEAs were more than 20% higher compared to the non-aggregated enzyme. amine transaminase enzyme immobilization cross-linked enzyme aggregates membrane microreactor flow biocatalysis amin transaminaza immobilizacija encimov zamreženi encimski agregati membranski mikroreaktor pretočna biokataliza true false false Angleški jezik Slovenski jezik Neznano 2022-07-12 11:25:22 2022-07-12 11:30:37 2025-04-16 12:52:31 0000-00-00 00:00:00 2022 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 CLEA_generation.xlsx CLEA_generation.xlsx CLEA generation excel file contains data from DLS particle size measurement and ATA and ATA-CLEA activity tests. Each Sheet in excel file has its own description of experimental procedures and results. 1 687E77E8FF421F70C617834A410FDB7D c48f190425b081789d749c1cca7d446bc6c322d16cd16dd69904ba27965a6685 267f4c3d-1df4-11ed-92af-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=159209 membrane_microreactor.xlsx membrane_microreactor.xlsx Membrane microreactor excel file contains data of biotransformations with ATA and ATA-CLEA in a membrane microreactor. Each Sheet in excel file has its own description of experimental procedures and results. 1 007283B136EF1B041BB260A1E4B589A1 d2d9938dc099c403562fdc4d9dcecc62dc7ea200a47af03585f216dc51d4a51a 80decc0d-1f89-11ed-92af-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=159265 litesizer_data.zip litesizer_data.zip Original DLS measurements. 1 62F374481EF795B24C704A1505AB716D ae05a41158b9d71c1d79d4904ff25a46a385007c44fb8f60cf26877d5a6e4d8e cba934fb-01da-11ed-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=158383 Activity_clea.zip Activity_clea.zip HPLC chromatograms for enzyme activity determination. 1 9CCFF1DA3674DAE8108B0384BF183E07 144286bcff727cebbf09de71f4e0716e47726a59b64919212f40046f75335a7d da19791e-01da-11ed-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=158384 SEM_ATA-CLEA.docx SEM_ATA-CLEA.docx SEM pictures of ATA-CLEAs 1 17D99919C8E5AFC31BC72EC55601FC26 6dda19ff10593b29fd744de7e4db2ac7b6288b2045c1079a0c7287eb64103426 0d9f4263-01db-11ed-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=158385 Fakulteta za kemijo in kemijsko tehnologijo Meritve in testi 0 0 0