20.500.12556/RUL-137960 Antioxidant and antimicrobial biofoil based on chitosan and Japanese knotweed (Fallopia japonica, Houtt.) rhizome bark extract A 70% ethanol(aq) extract of the rhizome bark of the invasive alien plant species Japanese knotweed (JKRB) with potent (in the range of vitamin C) and stable antioxidant activity was incorporated in 1% w/v into a chitosan biofoil, which was then characterized on a lab-scale. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay confirmed the antioxidant activity of the JKRB biofoil upon contact with the food simulants A, B, C, and D1 (measured half-maximal inhibitory concentrations—IC50) and supported the Folin–Ciocalteu assay result. The migration of the antioxidant marker, (−)-epicatechin, into all food simulants (A, B, C, D1, D2, and E) was quantified using liquid chromatography hyphenated to mass spectrometry (LC-MS). Calculations showed that 1 cm2 of JKRB biofoil provided antioxidant activity to ~0.5 L of liquid food upon 1 h of contact. The JKRB biofoil demonstrated antimicrobial activity against Gram-positive bacteria. The incorporation of JKRB into the chitosan biofoil resulted in improved tensile strength from 0.75 MPa to 1.81 MPa, while elongation decreased to 28%. JKRB biofoil’s lower moisture content compared to chitosan biofoil was attributed to the formation of hydrogen bonds between chitosan biofoil and JKRB compounds, further confirmed with attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The JKRB biofoil completely degraded in compost in 11 days. The future upscaled production of JKRB biofoil from biowastes for active packaging may support the fights against plastic waste, food waste, and the invasiveness of Japanese knotweed, while greatly contributing to the so-called ‘zero-waste’ strategy and the reduction in greenhouse gas emissions. Japanese knotweed rhizome bark extract chitosan antioxidant biofoil antimicrobial activity biodegradable material active packaging true false true Angleški jezik Ni določen Članek v reviji 2022-07-07 08:01:03 2022-07-08 11:37:20 2022-09-15 04:16:06 0000-00-00 00:00:00 2022 0 0 22 st. no. 6, art. 1200 Vol. 11 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579 2076-3921 10.3390/antiox11061200 112904195 Antioxidant-Golob.pdf Antioxidant-Golob.pdf 1 D8E3C3C6402859FCEA43A48D0C93A85A 07a8dfd597ce30679daf895399857075f2d23c941956058eb8f2fd5860425acf 2e09eec8-fea1-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=158252 https://www.mdpi.com/2076-3921/11/6/1200 1 f6f2176a-fdb9-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=158093 Veterinarska fakulteta 0 0 0