20.500.12556/RUL-137799 Influence of excipient composition on survival of vaginal lactobacilli in electrospun nanofibers The lack of appropriate delivery systems hinders the use of probiotics in the treatment of vaginal infections. Therefore, the development of a new delivery system for the local administration of vaginal probiotics is necessary. In this study, we selected three vaginal lactobacilli, i.e., Lactobacillus crispatus, Lactobacillus gasseri, and Lactobacillus jensenii, and incorporated them into nanofibers using electrospinning. Polyethylene oxide (PEO) was used as a carrier polymer to produce nanofibers. It was supplemented with alginate and sucrose selected from a group of carbohydrates for their growth-promoting effect on lactobacilli. The interaction between excipients and lactobacilli was evaluated thermally and spectroscopically. Bacterial survival in polymer solutions and in nanofibers immediately after electrospinning and after storage varied among species and was dependent on the formulation. Sucrose improved the survival in polymer solutions and preserved the viability of L. crispatus and L. jensenii immediately after electrospinning, and L. gasseri and L. jensenii during storage. Blending PEO with alginate did not improve species viability. However, the three lactobacilli in the nanofibers retained some viability after 56 days, indicating that composite multifunctional nanofibers can maintain the viability of vaginal lactobacilli and can be used as a potential solid delivery system for vaginal administration of probiotics. vaginal probiotics electrospinning nanofibers viability lyoprotectants carbohydrates delivery system nanovlakna dostavni sistemi true false true Angleški jezik Slovenski jezik Članek v reviji 2022-07-01 08:35:37 2022-07-01 08:35:42 2025-08-08 13:20:42 0000-00-00 00:00:00 2022 0 0 17 str. iss. 6, art. 1155 Vol. 14 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.017-022.513.2:615.015.14 1999-4923 10.3390/pharmaceutics14061155 110284547 pharmaceutics-14-01155.pdf pharmaceutics-14-01155.pdf 1 8ABEB7064345AC5A8852313A6E0E74E6 e1b945a8ef3c0a1bb6e06166df350e1cf8fa108dfd0816d9c9cb7438a446a345 48b56d0a-f931-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=157917 https://www.mdpi.com/1999-4923/14/6/1155 1 d2e72bb1-f907-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=157878 Fakulteta za farmacijo 0 0 0