20.500.12556/RUL-137745 Bioluminiscentne glive in aplikacije v biotehnologiji Bioluminescent fungi and applications in biotechnology Bioluminiscenca je pojav v naravi, ki nas privlači iz estetskih, znanstvenih in uporabnih razlogov. Eden od virov naravne bioluminiscence so določene vrste gliv, ki uporabljajo luciferinski mehanizem, za oddajanje svetlobe bodisi preko podgobja, gobe ali obojega. V grobem luciferinski mehanizem deluje tako, da je substrat oksidiran preko encimov, kar omogoči substratu prehod v vzbujeno stanje S1, ki ni stabilno, zato odda energijo v obliki svetlobe, da se ponovno vrne v stabilno S0 stanje. Ta mehanizem lahko v biotehnologiji uporabimo v ekotoksikologiji, kot reporterske mehanizme in za druge aplikacije. Raziskovali smo, kako različna kompleksnost substratov vpliva na svetilnost bioluminiscenčnih gliv. Zato smo uporabili štiri različne bioluminiscentne glivne kulture (Armillaria melllea, Panus conchatus, Panellus serotina, neznana glivna kultura) in štiri substrate (slamo, slamo s kavno usedlino, kokosova vlakna in bukov les). Glive smo inokulirali na substrate in spremljali njihovo svetilnost s senzorji svetlosti in kamero v rastni komori. Dodatno smo raziskali tudi vpliv temperature v rastni komori. Pri našem eksperimentu smo fluorescenco zaznali le pri glivi Armillaria mellea na bukovem lesu inkubirano pri temperaturi 10 ºC. Z našo raziskavo smo potrdili, da štorovka najbolje uspeva na bukovini in, da gliva potrebuje nižje temperature ozirom temperaturni šok, da zažene luciferinski cikel. Bioluminescence is a phenomenon in nature that attracts us from an esthetic, scientific and useful point of view. One of the sources of natural bioluminescence is some species of fungi that use the luciferin system to emit light from the mycelium, fruiting body, or both. In simple terms, the luciferin system involves the oxidation of a substrate by enzymes that bring the substrate to the first excited state S1, which is not stable. Energy is thus released in the form of light to bring the excited substrate back to the stable state S0. We can use this mechanism in ecotoxicology, as a reporter mechanism, and for other applications. We investigated how the complexity of the substrate affects the emitted light of bioluminescent fungi. For this purpose, we used straw, straw with coffee grounds, beech wood, coconut husks and four different fungal species (Armillaria mellea, Panus conchatus, Panellus serotina, unknown fungal species). We inoculated the substrates with fungi and monitored their luminosity with cameras and sensors in the growth chamber. We also tried varying the temperature in the growth chamber. We made Armillaria mellea to emit light at 10°C and beechwood substrate. We found that for Armillaria mellea the best conditions for the onset of the luciferin cycle are lower temperatures to induce shock and beech wood as substrate. glive bioluminiscenca luminiscenca substrat fungi bioluminescence luminescence substrate true false false [D. Valant] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2022-06-30 08:15:49 2022-06-30 08:15:53 2022-09-15 04:15:47 0000-00-00 00:00:00 2022 0 Ljubljana 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 606:582.28:604.4:535.37(043.2) 204659 114952451 23158.pdf 23158.pdf 1 EA9583667EDB739CC016A63F36E7E4C1 c0398bd783b4f72cd7c2924bc1e8fb9ca241c690e49075a9631f9d34a886d97e e3946ad9-f83b-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=157808 Biotehniška fakulteta 0 0 0