20.500.12556/RUL-137727 Skeletal-muscle-derived mesenchymal stem/stromal cells from patients with osteoarthritis show superior biological properties compared to bone-derived cells Mesenchymal stem/stromal cells (MSCs) are being exploited for patient-derived stem-cell therapies. As the biological properties of MSCs derived from skeletal muscle of osteoarthritis patients are poorly understood, the aim of this study was to compare muscle MSCs with well-recognized bone and bone marrow-derived MSCs from these patients. Paired samples of skeletal muscle and trabecular bone tissue were obtained from 21 patients with osteoarthritis. Isolated cells were compared using ex vivo immunophenotyping and detailed in vitro analyses. These included the colony forming unit fibroblast assay, growth kinetics, senescence, multilineage potential, immunophenotyping, and MSC marker gene expression profiling. Freshly isolated MSCs from muscle showed improved viability over bone-derived MSCs, with similar mesenchymal fraction. Muscle-derived MSCs showed superior clonogenicity, higher growth rates, and lower doubling times. Muscle-derived MSCs also showed superior osteogenic and myogenic properties and a positive correlation between CD271 expression and adipogenesis. Senescence rate as well as adipogenic and chondrogenic potentials were similar. Skeletal muscle-derived MSCs of osteoarthritis patients have superior clonogenicity and growth kinetics compared to bone-derived MSCs, making them a good candidate for autologous stem-cell therapies. Moreover, the positive correlation between CD271 and adipogenesis suggest that CD271 expressing muscle MSCs might contribute to muscle steatosis observed in osteoarthritis. mesenchymal stem/stromal cells osteoarthritis bone muscle CD271 artroza matične celice true false true Angleški jezik Slovenski jezik Članek v reviji 2022-06-29 12:09:34 2022-06-29 12:09:39 2022-09-28 08:16:49 0000-00-00 00:00:00 2019 0 0 12 str. Vol. 38, art. 101465 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 576.3:616.71-007.234 1873-5061 10.1016/j.scr.2019.101465 4717937 1-s2.0-S1873506119300959-main.pdf 1-s2.0-S1873506119300959-main.pdf 1 0392C6EB477628A06569092D6BD577A4 f4305a853fafe37549c32048e065095f744b5070721fac5cf81764f14cb22f83 dc0393bc-f793-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=157780 https://www.sciencedirect.com/science/article/pii/S1873506119300959 0 626dcd68-f793-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=157779 Fakulteta za farmacijo 0 0 0