20.500.12556/RUL-137633 Uporaba bioinformacijskih orodij za določanje tarčnih proteinov bioaktivnih komponent v živilih z anksiolitičnim delovanjem Bioinformatic tools for identification of target proteins of bioactive food components with anxiolitic activity Leta 2030 bi lahko na svetu trpelo okoli 900 mio ljudi zaradi težav z duševnim zdravjem. Anksioznost je druga najbolj pogosta psihična motnja, ki jo povzročata prekomeren stres in prehiter način življenja. Spoznamo jo po simptomih kot so: dušenje, pospešeno bitje srca, tresavica, živčni nemir in motnje spanja. Za lajšanje blažjih psihičnih motenj vse pogosteje posegamo po naravnih sredstvih. Namen dela je bil izbrani bioaktivni komponenti z anksiolitičnim učinkom iz živil, z bioinformacijskimi orodji napovedati proteinske tarče pri človeku. Po pregledu znanstvene literature smo izbrali flavonoid krizin, ki se nahaja v medu, gobah in zdravilnih rastlinah. Z inverznim sidranjem smo mu z orodjem ProBiS-Dock algoritem napovedali 25329 proteinskih tarč. Med prvimi 100 z najnižjo prosto vezno energijo smo identificirali 73 novih potencialnih tarč. S sekundarnim pregledom literature smo validirali metodo dela in opisali prvih 20 tarč ter njihovo fiziološko vlogo. Osredotočili smo se na dve tarči (PDE4B in KRAS), ki ju povezujejo z anksioznostjo, ter s programom PyMOL določili njune interakcije s krizinom. Sklepamo, da bi proteinska tarča PDE4B (phosphodiesterase 4B), glede na močno povezavo njenega gena PDE4B z anksioznostjo, lahko predstavljala pomembno odkritje pri uporabi krizina. Funkcionalna živila z bioaktivnimi komponentami, kot je krizin, bi lahko imela pomembno vlogo pri lajšanju anksioznosti na naraven način. In 2030, around 900 million people in the world could suffer from mental health disorders. Anxiety is the 2nd most common mental disorder, caused by stress and the fast-paced lifestyle. It is accompanied by characteristic symptoms such as suffocation, rapid heartbeat, tremor, nervous restlessness and sleep disturbances. Recently, there has been a growing interest in people alleviating milder forms of mental disorders with natural remedies. The purpose of this paper was to predict protein targets in human for selected bioactive compound with anxiolytic effect from the food with the use of bioinformational tools. Based on the scientific literature review, we selected the flavonoid chrysin, which is found mainly in honey, mushrooms and medicinal plants. With inverse docking, we predicted 25,329 protein targets with the ProBiS-Dock algorithm tool. Among the first 100 with the least free binding energy, we identified 73 new potential targets. With a secondary literature review, we validated the method of this work and described the first 20 targets and their physiological role. We focused on the two targets (PDE4B and KRAS) that have known association with anxiety and demonstrated their interactions with chrysin in the program PyMOL. Due to the strong link between PDE4B gene with anxiety, we hypothesized that PDE4B protein target (phosphodiesterase 4B), could represent an important discovery in the use of chrysin. Functional foods with bioactive compounds such as chrysin could play an important role in relieving anxiety in a natural way. funkcionalna hranila anksiolitični učinek živil bioaktivne komponente hrane krizin nutrigenomika epigenomika functional nutrients anxiolytic effect of food bioactive components of food chrysin nutrigenomics epigenomics true false false [Ž. Kotnik] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2022-06-24 07:15:49 2022-06-24 07:15:59 2022-09-15 04:15:33 0000-00-00 00:00:00 2022 0 Ljubljana 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 606:616.891.6:612.39(043.2) 204612 115108611 23159.pdf 23159.pdf 1 08B1CB38C359B4A53EF24D8EB84DC921 72f94ee318c4ec5dc0d253674ff85867c4730695cd012b7372adfa054cb1e247 8a2fdfc5-f37c-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=157663 Biotehniška fakulteta 0 0 0