20.500.12556/RUL-137625 2-acyl-1-aryl-6,7-dihydro-1H,5H-pyrazolo[1,2-a]pyrazole derivatives versatile fluorescent probes with remarkably large Stokes shift A series of thirteen 2-acyl-6,7-dihydro-1H,5H-pyrazolo[1,2-a]pyrazole derivatives were synthesized by using a copper-catalyzed azomethine-imine-alkyne cycloaddition as a key step. These bimane-related fluorescent compounds with λ$_{ex}$ = 338–400 nm and λ$_{em}$ = 522–644 nm exhibit interesting and promising optical properties, including a remarkably large Stokes shift (150–268 nm), positive solvatochromism, and strong dependence of emission intensity on pH in a range of pH 2–10. The benzotriazolide- and N-succinimidyl ester-functionalized compounds were successfully used as fluorescent probes for protein labelling. 6 7-dihydro-1H 5H-pyrazolo[1 2-a]pyrazoles benzotriazolides fluorescent imaging bioconjugation solvatochromism 6 7-dihidro-1H 5H-pirazolo[1 2-a]pirazoli benzotriazolidi fluorescentno označevanje biokonjugacija solvatokromija true false true Angleški jezik Slovenski jezik Članek v reviji 2022-06-23 14:30:49 2022-06-23 14:30:49 2022-09-19 12:18:38 0000-00-00 00:00:00 2022 0 0 13 str. art. 110224 Vol. 201 May 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 547.77 0143-7208 10.1016/j.dyepig.2022.110224 100346115 1-s2.0-S0143720822001462-main.pdf 1-s2.0-S0143720822001462-main.pdf 1 2A211972E1E1F1286CCC5559BF92FCD8 9e1c355b854b498cc873cf929e2ff62f9ab6673c1bcb8ea75c9a8c5f65c92e77 cf4a4b1e-f2f0-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=157653 https://www.sciencedirect.com/science/article/pii/S0143720822001462 1 1f2c3af7-f2f0-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=157651 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0