20.500.12556/RUL-137537 Knee and peri-knee tissues of post mortem donors are strategic sources of mesenchymal stem/stromal cells for regenerative procedures Tissues of post mortem donors represent valuable alternative sources for the isolation of primary cells with mesenchymal stem/stromal cell (MSC)-like properties. However, the properties of primary cells derived from different tissues and at different post mortem times are poorly recognized. Here, we aim to identify the optimal tissue source between three knee and peri-knee tissues for the isolation of primary cells with MSC-like properties, and to define the influence of the time post mortem on the properties of these cells. We harvested tissues from subchondral bone marrow, synovium and periosteum from 32 donors at various post mortem times. Primary cells were evaluated using detailed in vitro analyses, including colony formation, trilineage differentiation, immunophenotyping and skeletal stem cell marker-gene expression profiling. These data show that the primary cells with MSC-like properties isolated from these three tissues show no differences in their properties, except for higher expression of CD146 in bone-marrow cells. The success rate of the primary cell isolation is dependent on the post mortem time. However, synovium and periosteum cells isolated more than 48 h post mortem show improved osteogenic and chondrogenic potential. This study suggests that knee and peri-knee tissues from donors even 3 days post mortem are strategic sources of MSCs for regenerative procedures. mesenchymal stem/stromal cells post mortem donors knee peri-knee tissues bone and bone marrow synovium periosteum in vitro analysis human skeletal stem cell markers mezenhimske matične/stromalne celice darovalci ob smrti skeletne matične celice koleno perikolenska tkiva kostni mozeg sinovij periosteum in vitro analiza označevalci človeških skeletnih matičnih celic matične celice tkiva true false true Angleški jezik Slovenski jezik Članek v reviji 2022-06-21 11:37:21 2022-06-21 11:37:27 2022-09-15 04:15:21 0000-00-00 00:00:00 2022 0 0 21 str. iss. 6, art. 3170 Vol. 23 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602.9:576.7:611.728.3 1422-0067 10.3390/ijms23063170 103163395 ijms-23-03170-v2.pdf ijms-23-03170-v2.pdf 1 64D0779EA771D9EA060ECBFBD848430B db05c83edbe7eaa0375ae6b8fdd16ead9f844a5ea925cdb5ef15311cedaba3eb 0cdbfd40-f146-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=157522 https://www.mdpi.com/1422-0067/23/6/3170 1 9467300d-f145-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=157521 Medicinska fakulteta Fakulteta za farmacijo 0 0 0