20.500.12556/RUL-137449 Physiological concentrations of Cimicifuga racemosa extract do not affect expression of genes involved in estrogen biosynthesis and action in endometrial and ovarian cell lines In postmenopausal women, estrogen levels exclusively depend on local formation from the steroid precursors dehydroepiandrosterone sulfate and estrone sulfate (E1-S). Reduced estrogen levels are associated with menopausal symptoms. To mitigate these symptoms, more women nowadays choose medicine of natural origin, e.g., Cimicifuga racemosa (CR), instead of hormone replacement therapy, which is associated with an increased risk of breast cancer, stroke, and pulmonary embolism. Although CR treatment is considered safe, little is known about its effects on healthy endometrial and ovarian tissue and hormone-dependent malignancies, e.g., endometrial and ovarian cancers that arise during menopause. The aim of our study was to examine the effects of CR on the expression of genes encoding E1-S transporters and estrogen-related enzymes in control and cancerous endometrial and ovarian cell lines. CR affected the expression of genes encoding E1-S transporters and estrogen-related enzymes only at very high concentrations, whereas no changes were observed at physiological concentrations of CR. This suggests that CR does not exert estrogenic effects in endometrial and ovarian tissues and probably does not affect postmenopausal women’s risks of endometrial or ovarian cancer or the outcomes of endometrial and ovarian cancer patients. endometrial cancer Cimicifuga racemosa ovarian cancer steroid transporters rak endometrija Cimicifuga racemosa rak jajčnikov true false true Angleški jezik Slovenski jezik Članek v reviji 2022-06-17 08:52:11 2022-06-17 08:52:15 2022-09-15 04:15:09 0000-00-00 00:00:00 2022 0 0 19 str. iss. 4, art. 545 Vol. 12 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-006 2218-273X 10.3390/biom12040545 104049411 biomolecules-12-00545.pdf biomolecules-12-00545.pdf 1 FC74807EC33F4925D826E067B324AAC7 8f0d6769327b3bbf7891fdae1b0ab8c1abf143d84c8e5b347dbb334cfd763676 3c8735ac-ee0a-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=157393 https://www.mdpi.com/2218-273X/12/4/545 0 d7f6a591-ee09-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=157392 Medicinska fakulteta 0 0 0