20.500.12556/RUL-137443 A biomimetic porcine urothelial model for assessing Escherichia coli pathogenicity Urinary tract infections can be severe, sometimes fatal, diseases whose etiological pathogens are predominantly uropathogenic strains of E. coli (UPEC). To investigate the UPEC pathogenesis, several models have already been established with minor or major disadvantages. The aim was to develop a simple, fast, and inexpensive biomimetic in vitro model based on normal porcine urothelial (NPU) cells that are genetically and physiologically similar to human bladder urothelium and to perform basic studies of E. coli pathogenicity. Initially, the model was tested using a set of control E. coli strains and, subsequently, with human E. coli strains isolated either from patients with urinary infections or from the feces of healthy individuals. A drop in viability of NPU cells was used as a measure of the pathogenicity of the individual strain tested. To visualize the subcellular events, transmission and scanning electron microscopy was performed. The strains were tested for the presence of different virulence-associated genes, phylogroup, type of core lipid, O-serotype, and type of lipopolysaccharide and a statistical analysis of possible correlations between strains’ characteristics and the effect on the model was performed. Results showed that our model has the discriminatory power to distinguish pathogenic from non-pathogenic E. coli strains, and to identify new, potentially pathogenic strains. urothelium virulence-associated genes infection electron microscopy E. coli UPEC correlations pathogenicity groups primary culture infection assay prašičji urotelij Escherichia coli patogenost true false true Angleški jezik Slovenski jezik Članek v reviji 2022-06-17 07:52:26 2022-06-17 07:52:31 2022-09-15 04:15:08 0000-00-00 00:00:00 2022 0 0 16 str. iss. 4, art. 783 Vol. 10 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579 2076-2607 10.3390/microorganisms10040783 104312323 microorganisms-10-00783.pdf microorganisms-10-00783.pdf 1 5E2E3511CBAB762A9969B85570D98F86 986c509b26f395e4139194b2e3f20b4164f507d3d97a085778b4e9167ea62f45 5cab4bec-ee03-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=157376 https://www.mdpi.com/2076-2607/10/4/783 0 80f89e3d-ee01-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=157375 Biotehniška fakulteta Medicinska fakulteta 0 0 0