20.500.12556/RUL-137349 Evolutionary analysis of dipeptidyl peptidase I Human dipeptidyl peptidase I (DPPI) belongs to the family of papain-like cysteine peptidases. Its distinctive features are the unique exclusion domain which enables the eponymous activity and homotetramerization of DPPI, and its dependence on chloride ions for enzymatic activity. The oligomeric state of DPPI is unique in this family of predominantly monomeric peptidases. However, a distant DPPI ortholog from Plasmodium falciparum has been shown to be monomeric, indicating that the oligomeric state of DPPI varies between lineages. The aim of this work was to study the evolution of DPPI, with particular attention to the structural features that determine its characteristic enzymatic activity and preferences, and to reconstruct the evolution of its oligomerization. We analyzed fifty-seven selected sequences of DPPI and confirmed its presence in three lineages, namely, Amorphea (including animals and Amoebozoa), Alveolates and the metamonad Giardia. The amino acid residues that bind the chloride ion are highly conserved in all species, indicating that the dependence on chloride ions for activity is an evolutionarily conserved feature of DPPI. The number of N-glycosylation sites is significantly increased in animals, particularly vertebrates. Analysis of homology models and subunit contacts suggests that oligomerization is likely restricted to DPPIs in the Amorphea group. oligomerization molecular evolution cathepsin C oligomerizacija molekularna evolucija katepsin C true false true Angleški jezik Slovenski jezik Članek v reviji 2022-06-13 14:01:28 2022-06-13 14:01:29 2022-09-15 04:14:58 0000-00-00 00:00:00 2022 0 0 12 str. iss. 3, art. 1852 Vol. 23 Feb. 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.15 1661-6596 10.3390/ijms23031852 96531459 ijms-23-01852.pdf ijms-23-01852.pdf 1 BBD7FB607E1F306F4ABA5E6AF40ACC90 c58c1d7666f9c708da0e517f174a7572379dbfb3e7c102f21473a5eabdb93593 c34c783c-eb12-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=157275 https://www.mdpi.com/1422-0067/23/3/1852 0 63b8043c-eb10-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=157274 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0