20.500.12556/RUL-137348 Applicability of RPMI 2650 and Calu-3 cell models for evaluation of nasal formulations The RPMI 2650 and Calu-3 cell lines have been previously evaluated as models of the nasal and airway epithelial barrier, and they have demonstrated the potential to be used in drug permeation studies. However, limited data exist on the utilization of these two cell models for the assessment of nasal formulations. In our study, we tested these cell lines for the evaluation of in vitro permeation of intranasally administered drugs having a local and systemic effect from different solution- and suspension-based formulations to observe how the effects of formulations reflect on the measured in vitro drug permeability. Both models were shown to be sufficiently discriminative and able to reveal the effect of formulation compositions on drug permeability, as they demonstrated differences in the in vitro drug permeation comparable to the in vivo bioavailability. Good correlation with the available bioavailability data was also established for a limited number of drugs formulated as intranasal solutions. The investigated cell lines can be applied to the evaluation of in vitro permeation of intranasally administered drugs with a local and systemic effect from solution- and suspension-based formulations. RPMI 2650 cell line Calu-3 cell line nasal drug formulations nasal epithelium drug permeability true false true Angleški jezik Ni določen Članek v reviji 2022-06-13 13:32:21 2022-06-13 13:32:22 2022-09-15 04:14:58 0000-00-00 00:00:00 2022 0 0 19 str. iss. 2, art. 369 Vol. 14 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615 1999-4923 10.3390/pharmaceutics14020369 107048707 pharmaceutics-14-00369.pdf pharmaceutics-14-00369.pdf 1 A79BDF1F302D32E68B3BFC348C76664A 240a65f69ff1dc5d964fee07c8db538d84dc757af39f692dd96b5a3ed147c7d2 1d5783fc-eb0d-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=157271 https://www.mdpi.com/1999-4923/14/2/369 0 6a5e154e-eb0c-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=157269 Fakulteta za farmacijo Medicinska fakulteta 0 0 0